高等学校化学学报

高等学校化学学报 ›› 1995, Vol. 16 ›› Issue (10): 1569.

• 研究简报 • 上一篇    下一篇

新皮啡肽Ⅰ、Ⅱ类似物的设计与合成

胡晓愚1, 王锐2, 嘉庆1, 姬虹1   

  1. 1. 兰州大学应用有机化学国家重点实验室, 兰州, 730000;
    2. 兰州大学生物系, 兰州, 730000
  • 收稿日期:1994-10-25 修回日期:1995-03-18 出版日期:1995-10-24 发布日期:1995-10-24
  • 通讯作者: 王锐
  • 作者简介:胡晓愚,男,64岁,教授.
  • 基金资助:

    国家自然科学基金;国家教委优秀年轻教师基金;博士点专项基金

Design and Synthesis of Deltorphin Ⅰ, Ⅱ Analogues

HU Xiao-Yu1, WANG Rui2, JIA Qing1, JI Hong1   

  1. 1. National Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou, 730000;
    2. Department of Biology, Lanzhou University, Lanzhou, 730000
  • Received:1994-10-25 Revised:1995-03-18 Online:1995-10-24 Published:1995-10-24

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关键词: 新皮啡肽, 固相多肽合成, 类阿片肽, 类似物的设计

Abstract: Endogenous opioid deltorphinⅠ(DELⅠ),Ⅱ(DELⅠ)and their five analogues ([L-Ala2]DEL Ⅰ,[L-Ala2]DEL Ⅱ,[Val4,Asp5]DELⅠ,[Val4,Asp6]DELⅠ,[Gly4,Asp7]DELⅠ)were designed on a rational basis and were synthesized by solid phase peptide synthesis method.The synthesis was performed manually on BHAresin prepared by ourselves,DCC was used as coupling reagent and TFAas deboc reagent. Peptide-resin was cleavaged by HF, the crude peptides were obtained after lyophylization. The crude peptides were desalted on a sephadex Glo column, then the pure peptides were obtained by partitional chromatography on a sephadex G25 column. The purities of all synthetic peptides were greater than 95%, which were acquired by reversed HPLC analysis. The data we got from FABMS,AAA and sequence analysis were in agreement with the caculated values.

Key words: Deltorphins, Solid phase peptide synthesis, Opioid, Design of analogues

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