高等学校化学学报

高等学校化学学报 ›› 1994, Vol. 15 ›› Issue (9): 1309.

• 论文 • 上一篇    下一篇

用银和铅标记人白蛋白的离子选择电极电位法研究

史生华, 刘鹏, 郝彩丽, 冯彬, 王彦, 李伟   

  1. 西北大学化学系电分析化学研究所, 西安, 710069
  • 收稿日期:1993-09-13 修回日期:1994-06-12 出版日期:1994-09-24 发布日期:1994-09-24
  • 通讯作者: 史生华,男,59岁,教授.
  • 作者简介:史生华,男,59岁,教授.
  • 基金资助:

    国家自然科学基金

A Study of Human Albumin Labelled by Silver and Lead with Ion-Selective Electrodes

SHI Sheng-Hua, LIU Peng, HAO Cai-Li, FENG Bin, WANG Yan, LI Wei   

  1. Institute of Electroanalytical Chemistry of Chemical Department, Northwest University, Xian, 710069
  • Received:1993-09-13 Revised:1994-06-12 Online:1994-09-24 Published:1994-09-24

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被引次数

1

摘要： 用离子选择电极电位法研究了银和铅与人白蛋白(HSA)的配合平衡。明确证实银和铅都与HSA形成了单核配合物MA和MA2.Ag-HSA配合物的稳定性积β11=4.4×107,β12=1.0×1012;Pb-HSA配合物的β11=3.1×105、β12=2.0×109.根据HSA与人白蛋白羊抗血清(GAHA)的免疫反应,用银和铅分别标记HSA时,测定GAHA(效价1:40)的线性范围是0.2～2.0μL和0.4～2.8μL;而用银和铅分别标记GAHA时,测定HSA的线性范围是0～2.9×10-8mol/L和0～7.3×10-7mol/L.

关键词: 离子选择电极, 人白蛋白, 人白蛋白羊抗血清, 配合物稳定常数, 免疫分析

Abstract: The complex equilibria of Ag-human albumin (HSA) and Pb-HSAwere studied by ion-selective electrodes.It was clearly proved that both Ag and Pb with HSAformed mononuclear complexes MAand MA2 respectively.The stability products of Ag-HSAcomplexes are β11=4.4×107 and β12=1.0×1012;and that of Pb-HSAcomplexes are β11=3.1×105, β12=2.0×109.According to the immunochemical reaction of HSAwith albumin antiserum (GAHA), the linear range of determing GAHA(titer 1 : 40)is respectively 0.2-2.0μLand 0.4-2.8μLwhen HSAis labelled by Ag and Pb; moreover the linear range of determining HSAis respectively 0-2. 9×10-8mol/Land 0-7.3×10-7mol/Lwhen GAHAis labelled by Ag and Pb.

Key words: Ion-selective electrode, Human albumin, Albumin goat antiserum, Complex stability constant, Immunoassay

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