高等学校化学学报 ›› 1994, Vol. 15 ›› Issue (6): 824.

• 论文 • 上一篇    下一篇

显微荧光-阿达玛变换三维图象分析

梅二文, 曾小斌, 陈观铨, 曾云鹗   

  1. 武汉大学化学系, 武汉, 430072
  • 收稿日期:1993-09-07 修回日期:1994-01-06 出版日期:1994-06-24 发布日期:1994-06-24
  • 通讯作者: 梅二文,男,27岁,博士研究生.
  • 作者简介:梅二文,男,27岁,博士研究生.
  • 基金资助:

    国家自然科学基金

Microscope Fluorescence-Hadamard Transform Three-Dimensional Image Analysis

MEI Er-Wen, ZENG Xiao-Bin, CHEN Guan-Quan, ZENG Yun-E   

  1. Department of Chemistry, Wuhan University, Wuhan, 430072
  • Received:1993-09-07 Revised:1994-01-06 Online:1994-06-24 Published:1994-06-24

摘要: 把阿达玛变换多通道成象技术与荧光显微镜结合起来,首次成功地获得了细胞的阿达玛变换三维荧光图象,该图象能同时提供微小物体中某一物质的含量及分布等信息。

关键词: 阿达玛变换, 荧光显微镜, 图象分析

Abstract: This research combines Hadamard transform multiplexed image technique with fluorescence microscopy.By this method, a Hadamard transform microscope cell fluorescence image was obtained with one detector(PMT)for the first time.The image shows a lot of useful information including the appearance ofcell, the content and the distribution of some species in a cell at same time.The result shows the possibility of using Hadamard multiplexed image technique to obtain a cell fluorescence image with one detector.

Key words: Hadamard transform, Fluorescence microscope, Image analysis

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