高等学校化学学报

高等学校化学学报 ›› 1994, Vol. 15 ›› Issue (5): 672.

• 研究简报 • 上一篇    下一篇

Nafion-汞膜化学修饰电极测定GPT的研究

金利通, 叶建山, 史占玲, 方禹之   

  1. 华东师范大学化学系, 上海, 200062
  • 收稿日期:1993-05-18 修回日期:1993-10-04 出版日期:1994-05-24 发布日期:1994-05-24
  • 通讯作者: 金利通,男,56岁,教授.
  • 作者简介:金利通,男,56岁,教授.

A Study of Nafion-Mercury Film Chemically Modified Electrode for the Determination of GPT

JIN Li-Tong, YE Jian-Shan, SHI Zhan-Ling, FANG Yu-Zhi   

  1. Department of Chemistry, East China Normal University, Shanghai, 200062
  • Received:1993-05-18 Revised:1993-10-04 Online:1994-05-24 Published:1994-05-24

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关键词: Nafion-汞膜化学修饰电极, 谷丙转氨酶, &alpha, -酮戊二酸

Abstract: α-Ketoglutaric acid is determined by use of the Nafion-mercury film chemically modified electrode (Nafion-Hg film CME). The activity of GPTin human serum is determined indirectly. The linear range for the determination of α-ketoglutaric acid is 5. 0×10-3—1. 0×10-6 mol.L. The lowest detection is 5.0×10-7mol/L.The range for the determinaion of GPTis 2-200 U/L. This method has a high sensitivity and resists with disturbance.Good results were obtained for tests of human blood samples.

Key words: Nafion-mercury film chemically modified electrode, Glutamic pyruvic transaminase, α-Ketoglutaric acid

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