高等学校化学学报 ›› 1994, Vol. 15 ›› Issue (4): 499.

• 论文 • 上一篇    下一篇

开管柱固定化葡萄糖氧化酶反应器的制备及其在流动注射分析中的应用

邹公伟, 胡冠九, 文红梅, 王彦玲, 顾方晓   

  1. 南京大学化学系, 南京, 210008
  • 收稿日期:1993-04-22 修回日期:1993-10-03 出版日期:1994-04-24 发布日期:1994-04-24
  • 通讯作者: 邹公伟,男,52岁,副教授.
  • 作者简介:邹公伟,男,52岁,副教授.
  • 基金资助:

    国家自然科学基金

Preparation of Open Tubular Immobilized Glucose Oxidase Reactor and Its Application in Flow Injection Analysis

ZOU Gong-Wei, HU Guan-Jiu, WEN Hong-Mei, WANG Yan-Ling, GU Fang-Xiao   

  1. Department of Chemistry, Nanjing University, Nanjing, 210008
  • Received:1993-04-22 Revised:1993-10-03 Online:1994-04-24 Published:1994-04-24

摘要: 本文将葡萄糖氧化酶化学键合到粗糙化的玻璃毛细管内壁上,用开管柱固定化酶反应器-安培检测器进行葡萄糖的流动注射分析。讨论了流速对流动注射分析体系峰电流和分散度的影响,提出了测量开管柱固定化酶表观活力的方法。

关键词: 葡萄糖氧化酶, 开管柱固定化酶反应器, 流动注射分析

Abstract: Glucose oxidase was chemically bonded on the inner surface of glass capillary.The glucose was determined in the system of open tubular immobilized enzyme reactor-amperometric detector by flow injection analysis.The effect of flow rate on peak current and dispersion in FIAsystem was discussed.The method of measuring the apparent activity of the open tubular immobilized enzyme reactor was suggested.

Key words: Glucose oxidase, Open tubular immobilized enzyme reactor, Flow injection analysis

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