高等学校化学学报 ›› 1992, Vol. 13 ›› Issue (6): 813.

• 论文 • 上一篇    下一篇

细胞色素c氧化酶稳态动力学研究

赵建钢1, 杨同书2, 张学忠2, 程玉华2   

  1. 1. 中国康复研究中心康复医学基础研究所, 北京, 100077;
    2. 吉林大学酶工程实验室
  • 收稿日期:1991-06-11 出版日期:1992-06-24 发布日期:1992-06-24
  • 通讯作者: 赵建钢
  • 基金资助:

    吉林大学酶工程国家开放实验室基金

Studies on Steady-State Kinetics of Cytochrome c Oxidase

ZHAO Jian-gang1, YANG Tong-shu2, ZHANG Xue-zhong2, CHENG Yu-hua2   

  1. 1. Institute of Basic Medical Sciences, China Rehabilitation Research Center, Beijing, 100077;
    2. Enzyme Engineering Laboratory, Jilin University, Changchun, 130023
  • Received:1991-06-11 Online:1992-06-24 Published:1992-06-24

摘要: 应用分光光度计研究了细胞色素c氧化酶的稳态动力学。结果表明,酶与底物具有高低两个亲和相,催化反应受底物浓度调节,具有单底物多相反应动力学特点。

关键词: 细胞色素c氧化酶, 稳态动力学, 底物

Abstract: The steady-state kinetics of CCOwas studied by spectrophotometry.It is indicated that there are both higher and lower affinity phases when CCOreacts with its substrate ferrocy-tochrome c.The catalytic reaction is regulated by the concentration of the substrate and has a character of single substrate multiphasic kinetics.

Key words: Cytochrorne c Oxidase, Steady-state kinetics, Substrate

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