高等学校化学学报

高等学校化学学报 ›› 1991, Vol. 12 ›› Issue (7): 918.

• 论文 • 上一篇    下一篇

睾酮类兴奋剂测定中一些β-葡萄糖醛酸甙酶酶解能力的比较

张长久1, 张亦中1, 林强1, 叶荔1, 杨天乐1, 张霁2, 刘春胜2, 王慕邹2, 周同惠2, 杨则宜3   

  1. 1. 国家体育运动委员会运动医学研究所, 北京, 100029;
    2. 中国医学科学院药物研究所;
    3. 北京医科大学
  • 收稿日期:1990-03-07 出版日期:1991-07-24 发布日期:1991-07-24
  • 通讯作者: 张长久

Comparison of the Hydrolyzed Activity of β-Glucuronidases from Different Sources While Determining the Testosterone Level in Doping Control

Zhang Chang-jiu1, Zhang Yi-zhong1, Lin Qiang1, Ye Li1, Yang Tian-le1, Zhang Ji2, Liu Chun-sheng2, Wang Mu-zou2, Zhou Tong-hui2, Yang Ze-yi3   

  1. 1. National Research Institute of Sports Medicine, Beijing, 100029;
    2. Institute of Materia Medico, Chinese Academy of Medical Sciences, Beijing;
    3. Beijing Medical University, Beijing
  • Received:1990-03-07 Online:1991-07-24 Published:1991-07-24

PDF (PC)

摘要： 睾酮是国际奥委会规定禁用的99种兴奋剂之一,由于睾酮是内源性激素,因此不能靠定性来判断运动员是否服用了睾酮,在人体激素代谢中,产生睾酮差向异构体--表睾酮,在男性黄种人中睾酮与表睾酮浓度比约为1:1,白种人中略高一些,根据这一情况,国际奥委会规定,人尿中睾酮与表睾酮之间浓度比不得超过6:1。

关键词: 睾酮, 兴奋剂, &beta, -葡萄糖醛酸甙酶

Abstract: Selected Ion Monitor (SIM) of GC-MS is used to determine the amounts of testosterone (T), epitestosterone(ET) and their ratio in urine.The affection of five β-glucuronidases to the determination of Tlevel has been compared. It is figured out that both the Calf Enzyme (made in China) and the E. Coli(IX-A) from Sigma have strong activities while hydrolyzing the combined T, ETin urine. So they are fit to the determination of Tand ETlevel of urine in doping Control.

Key words: Testosterone, Doping, β-Glucuronidase

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