高等学校化学学报 ›› 1991, Vol. 12 ›› Issue (1): 36.

• 论文 • 上一篇    下一篇

化学发光法测定天麻素

庞志功, 汪宝琪, 刘万(冖斗)   

  1. 西安医科大学药学系, 710061
  • 收稿日期:1989-07-17 出版日期:1991-01-24 发布日期:1991-01-24
  • 通讯作者: 庞志功

Determination of Gastrodin with the Method of Chemilumincsccncc

Pang Zhi-gong, Wang Bao-qi, Liu Wan-jun   

  1. Department of Pharmacy, Xi'an Medical University, Xi'an, 710061
  • Received:1989-07-17 Online:1991-01-24 Published:1991-01-24

摘要: 天麻为兰科天麻属植物天麻GastrodiaelataBlume的块茎,性味甘平,有祛风定惊之功效,以天麻素为有效成分应用于临床.对天麻素的测定多用薄层层析-紫外分光光度法,而用化学发光法却未见报道.本文使用鲁米诺-过氧化氢-高锰酸钾发光体系,对天麻素进行了定量分析,取得了良好的效果.

关键词: 天麻素, 水解, 酶促反应, 化学发光

Abstract: Using Luminol-H2O2-KMnO4as the Luminescent system and H2O2generated from the hydrolysis and enzymatic reaction of gastrodin as the quantitative product, the luminescent intensity was measured. From the intensity of the product H2O2the content of gastrodin has been calculated.

Key words: Gastrodin, Hydrolysis, Enzymatic reaction, Chemiluminescence

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