高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

超声辅助自组装构建多功能铁-原卟啉纳米粒及多模态肿瘤治疗

李勇1，李燕3，杨景儿1，王柏萍1，尹君雅1，耿鹏1, 3，杨扬2，黄文权1, 2   

  1. 1. 三峡大学健康医学院，国家中医药管理局中药药理三级实验室

    2. 桂林医科大学，广西药物分子发现与成药性优化重点实验室 3. 三峡大学材料与化工学院

  • 收稿日期:2025-06-25 修回日期:2025-09-03 网络首发:2025-09-05 发布日期:2025-09-05
  • 通讯作者: 黄文权 E-mail:huangwenquan@ctgu.edu.cn
  • 基金资助:
    广西药物分子发现与成药性优化重点实验室开放课题资助(批准号：GKLDDO-2024-05)、湖北省自然科学基金项目(批准号：2024AFB059)和三峡大学高层次人才科研启动及平台建设经费(拔尖人才)(批准号：8220309)资助

Ultrasonically Assisted Self-assembly of Multifunctional Iron-protoporphyrin Nanoparticles and Multimodal Tumor Therapy

LI Yong1,  LI Yan3,  YANG Jinger1,  WANG Baiping1,  YIN Junya1  GENG Peng1, 3,  YANG Yang2,  HUANG Wenquan1, 2   

  1. 1. Third-grade Pharmacological Laboratory on Traditional Chinese Medicine(TCM), College of Medicine and Health Science, China Three Gorges University 2. Guangxi Key Laboratory of Drug Discovery and Optimization, School of Pharmacy, Guilin Medical University 3. College of Materials and Chemical Engineering, China Three Gorges University
  • Received:2025-06-25 Revised:2025-09-03 Online First:2025-09-05 Published:2025-09-05
  • Contact: Wenquan Huang E-mail:huangwenquan@ctgu.edu.cn
  • Supported by:
    Supported by the Project Program of Guangxi Key Laboratory of Drug Discovery and Optimization (No. GKLDDO-2024-05), the Natural Science Foundation of Hubei Province, China (No.2024AFB059), and the Scientific Research Start-up and Platform Construction of China Three Gorges University High-level Talents (top-notch talents) (No.8220309)

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摘要： 多模态协同治疗纳米材料对肿瘤精准治疗具有重要研究价值，但其传统制备方法复杂且协同治疗效果低.本研究采用超声辅助自组装策略合成了铁-原卟啉IX(PpIX)配位纳米粒(Fe-PpIX)，实现光/声/化学动力三位一体多模态肿瘤治疗. 采用TEM、XPS、FTIR对所制备纳米粒进行表征，表明Fe与PpIX羧基有效配位. 基于荧光光谱定量分析，结果显示在光和声协同处理下Fe-PpIX可有效产生ROS. 邻苯二胺/亚甲基蓝双探针检测发现，Fe-PpIX可催化内源性H2O2发生类Fenton反应生成羟基自由基，从而触发化学动力治疗. 采用L929和4T1细胞进行MTT实验，表明Fe-PpIX具有良好的生物相容性. 经光/声/化学协同治疗后，4T1肿瘤细胞的存活率显著下降，存活率降至最低(15.0%)，活/死细胞染色实验进一步证实了这一点. 本研究所制备纳米粒有望实现高效治疗，为理性设计多模态联合治疗的纳米粒提供新策略.

关键词: 自组装, 多模态协同治疗, 原卟啉PpIX, 光动力, 声动力

Abstract: Multimodal synergistic therapeutic nanomaterials exhibit significant research value for precision tumor therapy. However, the traditional preparation process is complex and the therapeutic efficacy of synergistic therapy remains suboptimal. In this study, an ultrasonically assisted self-assembly strategy is developed to synthesize iron-protoporphyrin IX (PpIX) coordination particle (Fe-PpIX). This approach achieves a three-in-one multimodal tumor therapy through photodynamic/sonodynamic/chemodynamic therapy. The prepared nanoparticles were characterized by TEM, XPS and FTIR, which indicated that Fe was successfully coordinated with PpIX by carboxyl groups. The quantitative evaluation of fluorescence spectroscopy demonstrated the efficient ROS generation of Fe-PpIX under photodynamic and sonodynamic conditions. The o-phenylenediamine/methylene blue dual probes further revealed that Fe-PpIX can catalyze the H2O2, which produce hydroxyl radicals by Fenton-like reaction. The MTT assays of L929 and 4T1 cells indicate that Fe-PpIX possesses good biocompatibility. After light/sound synergistic therapy, the survival rate of 4T1 tumor cells decreased significantly, and the survival rate decreased to the lowest (15.0%). This was further confirmed by live/dead cell staining experiments. The developed nanoparticles exhibit a valuable potential for achieving highly efficient therapy, providing a promising strategy for the rational design of multimodal therapeutic nanomaterials.

Key words: Self-assembly; Multimodal synergistic therapy, Protoporphyrin IX, Photodynamic, Sonodynamic

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