高等学校化学学报

高等学校化学学报

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基于核酸的蛋白标记技术在膜受体可视化分析中的应用

陈 珊1,2 ，卓育妃1 ，李婧影1   

  1. 1.福州大学化学学院 2.闽江学院地理与海洋学院
  • 收稿日期:2024-12-30 修回日期:2025-02-14 出版日期:2025-02-26 发布日期:2025-02-26
  • 通讯作者: 李婧影 E-mail:lijingying@fzu.edu.cn
  • 基金资助:
    福建省自然科学基金（批准号：2022J05240）、福建省杰出青年基金（批准号：2023J06016）和国家自然科学基金（批准号： 22207049，22474022）资助

Nucleic acid-based protein labeling tools for visualization of membrane receptors

CHEN Shan1,2, ZHUO Yufei1, LI Jingying1   

  1. 1.College of Chemistry, Fuzhou University 2. College of Geography and Oceanography, Minjiang University
  • Received:2024-12-30 Revised:2025-02-14 Online:2025-02-26 Published:2025-02-26
  • Contact: Jing-Ying LI E-mail:lijingying@fzu.edu.cn
  • Supported by:
    Supported by the Natural Science Foundation of Fujian Province, China(No. 2022J05240), the Outstanding Youth Foundation of Fujian Province, China(No. 2023J06016) and the National Natural Science Foundation of China(Nos. 22207049, 22474022)

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摘要： 细胞通讯等动态复杂的生命活动需要多种膜受体的参与。核酸探针是开发膜受体可视化分析策略的重要分子工具。本文从非共价靶向识别和共价偶联两种策略类型总结了基于核酸的蛋白标记技术。系统介绍了这些标记技术在膜受体表达水平、糖型以及相互作用等重要分子信息的可视化分析方面取得的最新研究进展，并对该领域的挑战和未来发展方向进行了阐述和展望。

关键词: 膜受体；核酸探针；蛋白标记；成像 

Abstract: Dynamic and complex cellular activities, such as cell communication and signal transduction, rely on the participation of various membrane receptors. Nucleic acid are important tools for developing membrane receptor visualization strategies in live cells. In this review, protein labeling techniques based on nucleic acid probes are summarized from two aspects: non-covalent target recognition and covalent coupling. The latest research progress of these labeling techniques in visualization of important molecular information, such as membrane receptor expression, glycoform and protein-protein interactions, was systematically reviewed. The existing challenges and future development in this field were discussed and prospected.

Key words: Membrane receptor, Nucleic acid probe, Protein labeling, Visualization 

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