黄豆黄素与黄豆黄苷吸收光谱和荧光光谱的比较研究

doi:10.7503/cjcu20180421

摘要/Abstract

摘要：

研究了黄豆黄素和黄豆黄苷在不同pH条件下的吸收光谱和荧光光谱, 从分子结构的角度解释了二者呈现不同光谱特征的原因. 黄豆黄素分子基本无荧光. 在弱碱性时, 黄豆黄素分子发生7-OH质子的电离, 导致吸收光谱中320 nm的吸收峰红移至348 nm. 采用pH-光度法测得7-OH质子的电离常数pK_a1=7.08±0.04. 黄豆黄素一价阴离子呈现较强荧光, 最大激发和发射波长λ_ex/λ_em分别为334 nm/464 nm, 荧光量子产率为0.049. 在碱性溶液中, 黄豆黄素4'-OH质子电离, 导致吸收光谱中254 nm的吸收峰红移至260 nm, 电离常数pK_a2=9.96±0.01. 黄豆黄苷分子基本无荧光. 在碱性条件下, 黄豆黄苷分子的4'-OH质子发生电离, 导致吸收光谱中256 nm的吸收峰红移至 280 nm, 电离常数pK_a=9.81±0.03. 黄豆黄苷阴离子基本无荧光, 但在热碱性条件下发生γ-吡喃酮环裂解反应而产生较强荧光, λ_ex/λ_em为288 nm/388 nm, 裂解产物的荧光量子产率为0.056. 虽然, 黄豆黄苷与黄豆黄素是苷与苷元的关系, 但黄豆黄苷不能在热碱性条件下通过糖苷水解转变为黄豆黄素, 二者的荧光增强机理存在本质不同.

关键词: 异黄酮, 黄豆黄素, 黄豆黄苷, 裂解反应, 荧光增强

Abstract:

The absorption and fluorescence spectra of glycitein and glycitin in aqueous solutions with different pH values were investigated in detail, and the reasons why the two presented different spectral characteristics were explained in the viewpoint of molecular structure. The molecular form of glycitein is essentially no fluorescence. Under weak alkaline condition, the 7-OH proton ionization causes a redshift of the absorbance peak at 320 nm to 348 nm. The proton ionization constant is measured to be pK_a1=7.08±0.04, by a pH-photometric method. The univalent-anion form of glycitein exhibit a fairly strong fluorescence with maximum excitation and emission wavelengths(λ_ex/λ_em) of 334 nm/464 nm, and the fluorescence quantum yield is measured to be 0.049. In alkaline solutions, the ionization of 4'-OH proton of glycitein causes a redshift of the absorbance peak at 254 nm to 260 nm, the ionization constant is pK_a2=9.96±0.01. The molecular form of glycitin has almost no fluorescence. Under alkaline conditions, the ionization of 4'-OH proton causes a redshift of the absorbance peak at 256 nm to 280 nm, with pK_a=9.81±0.03. The anion form of glycitin has almost no fluorescence, but the cleavage reaction of γ-pyrone ketone ring occurs under hot and alkaline conditions and produces a fairly strong fluorescence, with λ_ex/λ_em of 288 nm/388 nm, and the fluorescence quantum yield of the cleavage reaction product is 0.056. Although the relationship between glycitin and glycitein is as that of glycoside and aglycone, but glycitin cannot be converted to glycitein by means of hydrolysis of glycoside under hot alkaline conditions. The fluorescence enhancement mechanisms of these two are essentially different.

Key words: Isoflavone, Glycitein, Glycitin, Cleavage reaction, Fluorescence enhancement

TrendMD:

李文红, 王丹阳, 曹津津, 魏永巨. 黄豆黄素与黄豆黄苷吸收光谱和荧光光谱的比较研究. 高等学校化学学报, 2019, 40(1): 47.

LI Wenhong,WANG Danyang,CAO Jinjin,WEI Yongju. Comparative Study of Absorption and Fluorescence Spectra of Glycitein and Glycitin^†. Chem. J. Chinese Universities, 2019, 40(1): 47.

图/表 14

Fig.1 Molecular structures of glycitein(A) and glycitin(B)

Fig.2 Absorption spectra of glycitein aqueous solutions at different pH values(A, C) and the relationship between absorbance and pH value(B, D)

Scheme 1 Proton dissociation reaction of glycitein Volume fraction of MeOH: 10%. (B) 6.56 μg/mL, 254 nm; (D) 6.76 μg/mL, 238 nm.

Table 1 Determination of pKa1 of glycitein*

pH	A	pK_a1	Average(pK_a1)
6.85	0.2824	7.14	7.08±0.04
6.94	0.3206	7.10	7.08±0.04
7.00	0.3475	7.08	7.08±0.04
7.04	0.3856	7.01	7.08±0.04

Table 2 Determination of pKa2 of glycitein*

pH	A	pK_a2	Average(pK_a2)
9.74	0.5511	9.95	9.96±0.01
9.86	0.5423	9.96	9.96±0.01
10.02	0.5311	9.97	9.96±0.01
10.21	0.5161	9.96	9.96±0.01

Fig.3 Fluorescence spectra of glycitein aqueous solutions at different pH values(A) and relationship between fluorescence intensity and pH value(B) c: 1.56 μg/mL, volume fraction of MeOH: 10%, λex/λem: 347 nm/456 nm.

Scheme 9 Cleavage reaction of glycitein

Fig.4 Absorption spectra of glycitin aqueous solutions at different pH values(A) and relationship between absorbance and pH value(B) c: 8.20 μg/mL, volume fraction of MeOH: 10%, λ=280 nm.

Scheme 3 Proton dissociation reaction of glycitin

Table 3 Determination of pKa of glycitin*

pH	A	pK_a	Average(pK_a)
9.63	0.3114	9.85	9.81±0.03
9.79	0.3342	9.87	9.81±0.03
9.90	0.3633	9.80	9.81±0.03
10.12	0.3946	9.81	9.81±0.03

Fig.5 Fluorescence spectra of glycitin alkaline solutions at different pH values(A) and influence of pH on fluorescence intensity(B) c: 1.68 μg/mL, volume fraction of MeOH: 10%, heat for 0.5 h at 100 ℃, λex/λem: 307 nm/415 nm.

Scheme 4 Cleavage and hydrolysis reaction of glycitin

Fig.6 Influence of methanol on fluorescence intensity of glyciteinc: 1.56 μg/mL, pH=8.8, λex/λem: 347 nm/456 nm.

Fig.7 Relationship between fluorescence intensity and heating temperaturec: 1.68 μg/mL, volume fraction of MeOH: 10%, heat for 0.5 h, pH=13.2, λex/λem: 307 nm/415 nm.

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