高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (9): 1655.doi: 10.7503/cjcu20160407

• 有机化学 • 上一篇    下一篇

DNA三角双锥结构的放射性标记

李剑波(), 王雪梅, 包宝亮, 何玉林, 王相成, 张国建, 白侠, 刘磊   

  1. 内蒙古医科大学附属医院核医学科, 呼和浩特 010050
  • 收稿日期:2016-06-06 出版日期:2016-09-10 发布日期:2016-08-23
  • 作者简介:联系人简介: 李剑波, 男, 博士, 助理研究员, 主要从事分子影像探针方面的研究. E-mail:lijianbo_1235@163.com
  • 基金资助:
    国家自然科学基金(批准号: 81360227)和内蒙古自治区自然科学基金[批准号: 2015BS(LH)0803]资助

Radiolabeling of DNA Bipyramid Nanostructures

LI Jianbo*(), WANG Xuemei, BAO Baoliang, HE Yulin, WANG Xiangcheng, ZHANG Guojian, BAI Xia, LIU Lei   

  1. Department of Nuclear Medicine, Inner Mongolia Medical University Affiliated Hospital, Hohhot 010050, China
  • Received:2016-06-06 Online:2016-09-10 Published:2016-08-23
  • Contact: LI Jianbo E-mail:lijianbo_1235@163.com
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.81360227) and the Natural Science Foundation of Inner Mongolia, China[No.2015BS(LH)0803]

摘要:

分别采用点击化学方法对DNA三角双锥结构进行氟-18标记研究, 采用二氯亚锡还原法对DNA三角双锥结构进行锝-99m标记研究. 结果表明, 点击化学方法不适用于DNA三角双锥结构的氟-18标记; 而采用二氯亚锡还原法则制得了以DNA三角双锥结构为载体的分子影像探针 99mTc-DBNs.

关键词: DNA三角双锥结构, 放射性标记, 氟-18, 锝-99m

Abstract:

DNA bipyramid nanostructures were radiolabeled with fluorine-18 through click chemistry. Reactant concentration, catalyst dosage, reaction time, temperatureand reaction frequency in the CuAAC reaction were studied. DNA bipyramid nanostructures were also radiolabeled with technetium-99m through stannous chloride lableling method. The results showed that it was not a good idea to radiolabel DNA bipyramid nanostructures with fluorine-18 through click chemistry. At the same time 99mTc-DBNs were successfully prepared. Radioactive molecular imaging probes would be obtained based on the nanoplatforms of DNA bipyramid nanostructures.

Key words: DNA bipyramid nanostructure, Radiolabeling, Fluorine-18, Technetium-99m

中图分类号: 

TrendMD: