呼气中挥发性硫化物的质子转移反应质谱在线检测

doi:10.7503/cjcu20140794

高等学校化学学报 ›› 2015, Vol. 36 ›› Issue (2): 236.doi: 10.7503/cjcu20140794

呼气中挥发性硫化物的质子转移反应质谱在线检测

沈成银¹, 王鸿梅², 黄超群¹, 陆燕¹, 夏磊¹, 陈小景¹, 王宏志¹(), 储焰南¹()

1. 中国科学院合肥物质科学研究院医学物理与技术中心/肿瘤医院, 合肥 230031
2. 中国科学院安徽光学精密机械研究所, 合肥 230031

收稿日期:2014-09-02 出版日期:2015-02-10 发布日期:2015-01-15
作者简介:联系人简介: 王宏志, 男, 副主任医师, 主要从事恶性肿瘤诊疗方法研究. E-mail: wanghz@hfcas.ac.cn 储焰南, 男, 博士, 研究员, 博士生导师, 主要从事医用/环境光谱质谱研究. E-mail: ychu@aiofm.ac.cn
基金资助:
国家自然科学基金(批准号: 21107112)、中国科学院知识创新工程重要方向项目(批准号: KGCX-YW-917)、安徽省科技攻关项目(批准号: 1301042095)和合肥物质科学技术中心创新项目培育基金(批准号: 2012FXCX009, 2014FXCX007)资助

On-line Detection of Volatile Sulfur Compounds in Breath Gas by Proton Transfer Reaction Mass Spectrometry^†

SHEN Chengyin¹, WANG Hongmei², HUANG Chaoqun¹, LU Yan¹, XIA Lei¹, CHEN Xiaojing¹, WANG Hongzhi^1,^*(), CHU Yannan^1,^*()

1. Center of Medical Physics and Technology & Cancer Hospital, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China
2. Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China

Received:2014-09-02 Online:2015-02-10 Published:2015-01-15
Contact: WANG Hongzhi,CHU Yannan E-mail:wanghz@hfcas.ac.cn;ychu@aiofm.ac.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.21107112), the Knowledge Innovation Program of the Chinese Academy of Sciences(No KGCX-YW-917), the Anhui Provincial Program for Science and Technology Development, China(No.1301042095) and the Innovative Program of Development Foundation of Hefei Center of Physical Science and Technology, China(Nos.2012FXCX009, 2014FXCX007)

摘要/Abstract

摘要：

利用自主研制的高灵敏呼气检测质子转移反应质谱(PTR-MS), 对一名口臭受试者的口腔吹出气体、鼻子呼出气体和口腔内气体分别进行多组分实时监测, 发现了该受试者呼气中3种引起口臭的挥发性硫化物(VSCs)的来源并不相同, 其中甲硫醇主要来源于口腔, 硫化氢和二甲基硫则主要来源于肺或呼吸道. 本研究不仅发现了口腔内气体在PTR-MS呼气监测过程中的特征变化趋势, 还分析了呼出气体中3种VSCs的来源, 对于呼气的正确采样和检测具有重要的指导意义.

关键词: 呼气, 挥发性硫化物, 质子转移反应质谱, 挥发性有机物, 在线检测

Abstract:

To develop a kind of noninvasive method of breath diagnosis in diseases, much attention has been paid to the study of the relation between diseases and volatile organic compounds in human breath gas. However, the gas in oral cavity was usually ignored in many studies of breath gas. The bad breath odor of a participant was studied by a home-made proton transfer reaction mass spectrometer(PTR-MS). The breath via the mouth, breath via the nose and the air in the mouth cavity were monitored with the mode of multiple ion detect scans. The results show that three different volatile sulfur compounds that cause bad breath odor should be attributed to different sources. The source of methyl mercaptan in breath is oral cavity, and the source of hydrogen sulphide and dimethylsulfide in breath is lung or respiratory tract. The related result is very important to sampling and detection of breath gas.

Key words: Breath gas, Volatile sulfur compound, Proton transfer reaction mass spectrometry, Volatile organic compound, On-line detection

中图分类号:

O657.63
O659

TrendMD:

沈成银, 王鸿梅, 黄超群, 陆燕, 夏磊, 陈小景, 王宏志, 储焰南. 呼气中挥发性硫化物的质子转移反应质谱在线检测. 高等学校化学学报, 2015, 36(2): 236.

SHEN Chengyin, WANG Hongmei, HUANG Chaoqun, LU Yan, XIA Lei, CHEN Xiaojing, WANG Hongzhi, CHU Yannan. On-line Detection of Volatile Sulfur Compounds in Breath Gas by Proton Transfer Reaction Mass Spectrometry^†. Chem. J. Chinese Universities, 2015, 36(2): 236.

图/表 4

Fig.1 Schematic diagram of the sampling of breath via the mouth(A), breath via the nose(B) and the air in the mouth cavity(C)

Fig.2 Monitoring VOCs of breath via mouth by PTR-MS (A) Three kinds of VOCs from five mouth breathings; (B) two kinds of VOCs from the second mouth breathing in (A).

Fig.3 Monitoring result of VOCs from mouth breathing, nose breathing and the gas in oral cavity Monitored ions at m/z 21, 42, 49 and 59.

Fig.4 Monitoring result of VOCs from mouth breathing, nose breathing and the gas in oral cavity Monitored ions at m/z 35 and 63.

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呼气中挥发性硫化物的质子转移反应质谱在线检测

On-line Detection of Volatile Sulfur Compounds in Breath Gas by Proton Transfer Reaction Mass Spectrometry^†

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呼气中挥发性硫化物的质子转移反应质谱在线检测

On-line Detection of Volatile Sulfur Compounds in Breath Gas by Proton Transfer Reaction Mass Spectrometry†

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On-line Detection of Volatile Sulfur Compounds in Breath Gas by Proton Transfer Reaction Mass Spectrometry^†