纳米金-卟啉复合传感阵列快速识别肺癌患者呼气中的有机小分子

doi:10.7503/cjcu20140648

高等学校化学学报 ›› 2015, Vol. 36 ›› Issue (2): 241.doi: 10.7503/cjcu20140648

纳米金-卟啉复合传感阵列快速识别肺癌患者呼气中的有机小分子

徐永红¹, 霍丹群¹(), 法焕宝², 杨眉¹, 侯长军¹

1. 生物流变科学与技术教育部重点实验室, 重庆大学生物工程学院, 重庆 400044
2. 重庆大学化学化工学院, 重庆 400030

收稿日期:2014-07-14 出版日期:2015-02-10 发布日期:2015-01-22
作者简介:联系人简介: 霍丹群, 女, 博士, 教授, 博士生导师, 主要从事卟啉生化传感器研究. E-mail: huodq@cqu.edu.cn
基金资助:
国家自然科学基金(批准号: 81171414, 81271930)、国家科技支撑计划项目(批准号: 2012BAI19B03)、重庆市研究生科研创新项目(批准号: CYB14028)和重庆大学大型仪器设备开放基金资助

Sensor Array Based on MTPP-AuNPs Compounds for Fast Identification of Organic Small Molecules in Exhaled Breath of Lung Cancer Patients^†

XU Yonghong¹, HUO Danqun^1,^*(), FA Huanbao², YANG Mei¹, HOU Changjun^1,^*

1. Key Laboratory of Biorheological Science and Technology, Ministry of Education, School of Bioengineering, Chongqing University, Chongqing 400044, China
2. School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China

Received:2014-07-14 Online:2015-02-10 Published:2015-01-22
Contact: HUO Danqun,HOU Changjun E-mail:huodq@cqu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(Nos 81171414, 81271930), the Key Technologies R&D Program of China(No.2012BAI19B03), Chongqing Graduate Student Research Innovation Project, China(No CYB14028) and the Sharing Fund of Chongqing University’s Large Equipment, China

摘要/Abstract

摘要：

合成了小粒径纳米金作为卟啉保护剂, 以纳米金-卟啉化合物和pH指示剂共同构建6×6传感阵列, 将其用于可视化检测12种肺癌患者呼出气体中的有机小分子(OSMs), 设置了3个浓度梯度且每个样品重复5次. 实验结果显示, 纳米金的引入可显著提高阵列的稳定性和灵敏度; 聚类分析结果表明, 5次平行实验的结果优先聚为一簇, 结构相似的目标物随后聚在一起; 差谱图显示该方法对不同种类、不同浓度的OSMs具有良好的定性及半定量分析能力. 该方法可快速识别OSMs且具有肺癌筛查的潜在应用前景.

关键词: 阵列传感器, 纳米金-卟啉, 快速识别, 呼气中有机小分子, 肺癌

Abstract:

The smaller gold nanoparticles(AuNPs) were synthesized and used as a protectant for metal tetraphenylporphyrins(MTPP), and a 6×6 sensor array was constructed together by AuNPs-MTPP compounds and pH indicators to detect 12 organic small molecules(OSMs) in exhaled breath of lung cancer patients with three concentration gradients, and each test was repeated five times. The protective effect showed that the stability and sensitivity of the array were significantly enhanced due to the introduction of AuNPs; hierarchical cluster analysis(HCA) indicated that the results of five parallel experiments gathered into a cluster firstly, and target objects with similar structure were gotten together subsequently; digital color difference maps of OSMs with different kinds and concentrations visually displayed that the proposed method had good capability for qualitative and semi-quantitative analysis. The method can be used for fast identification of OSMs and has potential application prospect in lung cancer screening.

Key words: Array sensor, Gold nanoparticles-metal tetraphenylporphyrins(AuNPs-MTPP), Rapid identification, Organic small molecules(OSMs) in exhaled breath, Lung cancer

中图分类号:

O657

TrendMD:

徐永红, 霍丹群, 法焕宝, 杨眉, 侯长军. 纳米金-卟啉复合传感阵列快速识别肺癌患者呼气中的有机小分子. 高等学校化学学报, 2015, 36(2): 241.

XU Yonghong, HUO Danqun, FA Huanbao, YANG Mei, HOU Changjun. Sensor Array Based on MTPP-AuNPs Compounds for Fast Identification of Organic Small Molecules in Exhaled Breath of Lung Cancer Patients^†. Chem. J. Chinese Universities, 2015, 36(2): 241.

图/表 7

Fig.1 Gas flow diagrams of detection system(A), structure schematic diagram of chamber(B) and response mechanism(C) of sensor’s response to gas mixture

Table 1 Temperatures, saturated vapor pressures, volumes and RSD of 2.16 mg/m3 tested objects

Category	Compound	Temperature/℃	Pressure/Pa	Volume/mL	RSD(%)
Hydrocarbon	Pentane	20	57300	0.04	3.1
	Heptane	20	4800	0.52	2.8
	Undecane	20	140	17.86	3.2
	Isoprene	15.4	53320	0.05	3.0
Alcohols	Methanol	20	12800	0.20	3.3
	Butanol	20	700	3.62	3.6
Aldoketones	Hexanal	20	1150	2.20	3.7
	Heptanal	25	400	6.33	4.9
	Acetone	20	23300	0.11	3.4
Benzenes	Benzene	27	13820	0.18	3.3
	Toluene	27	4180	0.61	4.5
	Styrene	20	600	4.22	5.0

Fig.2 UV-Vis absorbance spectra(A), digital photos(B) of AuNPs(a, a) and HAuCl4·4H2O(b, b), fluorescence spectrum(C) and SEM image(D) of AuNPs in aqueous solution

Fig.3 Protective effects of AuNPs, array(for 2.16 mg/m3 benzene) stored under N2 during different lay-out time Digital color difference maps with/without protection of AuNPs(A) and the Euclidean distance with(B)/without(C) protection of AuNPs.

Scheme 1 Interaction between MTPP and AuNPs via non-bonding electrons on nitrogens of MTPP

Fig.4 Digital color difference maps of AuNPs-MTPP sensor array for 12 OSMs(2.16 mg/m3)

Fig.5 Hierarchical cluster analysis for 12 OSMs with 2.16 mg/m3 Insets are difference maps of hexanal at three concentrations.

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纳米金-卟啉复合传感阵列快速识别肺癌患者呼气中的有机小分子

Sensor Array Based on MTPP-AuNPs Compounds for Fast Identification of Organic Small Molecules in Exhaled Breath of Lung Cancer Patients^†

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纳米金-卟啉复合传感阵列快速识别肺癌患者呼气中的有机小分子

Sensor Array Based on MTPP-AuNPs Compounds for Fast Identification of Organic Small Molecules in Exhaled Breath of Lung Cancer Patients†

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Sensor Array Based on MTPP-AuNPs Compounds for Fast Identification of Organic Small Molecules in Exhaled Breath of Lung Cancer Patients^†