高等学校化学学报

高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (8): 1840.doi: 10.7503/cjcu20130427

• 分析化学 • 上一篇    下一篇

羟基多环芳烃的电喷雾电离离子阱串联质谱检测

李雪1,2, 方小伟2, 李银萍1, 陈焕文2   

  1. 1. 上海大学环境与化学工程学院, 环境污染与健康研究所, 上海 200444;
    2. 东华理工大学江西省质谱科学与仪器重点实验室, 南昌 330013
  • 收稿日期:2013-05-07 出版日期:2013-08-10 发布日期:2013-07-19
  • 通讯作者: 李雪,女,博士,助理研究员,主要从事新兴有机污染物分析方法及其健康风险评价研究.E-mail:zlpamylee@gmail.com;陈焕文,男,博士,教授,博士生导师,主要从事有机质谱分析和仪器研究.E-mail:chw8868@gmail.com E-mail:zlpamylee@gmail.com;chw8868@gmail.com
  • 基金资助:

    国家自然科学基金(批准号: 21107066); 国家重大科学仪器设备开发专项(批准号: 2011YQ170067); 上海高校青年教师培养资助计划和上海大学创新基金资助.

Detection of Hydroxylated Metabolites of Polycyclic Aromatic Hydrocarbons by Electrospray Ionization Ion Trap Tandem Mass Spectrometry

LI Xue1,2, FANG Xiao-Wei2, LI Yin-Ping1, CHEN Huan-Wen2   

  1. 1. Institute of Environmental Pollution and Health, School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;
    2. Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China Institute of Technology, Nanchang 330013, China
  • Received:2013-05-07 Online:2013-08-10 Published:2013-07-19

PDF (PC)

被引次数

3

摘要：

采用电喷雾电离离子阱串联质谱检测了1-/2-羟基萘、 2-羟基芴、 2-/3-/4-/9-羟基菲、 6-羟基屈和3-羟基苯并[a]芘等9种不同环数的羟基多环芳烃(OH-PAHs, 2~5环), 考察了碰撞诱导解离操作参数活化值Q和相对碰撞能量对羟基多环芳烃各单体碎片离子产率的影响. 通过优化活化值Q和相对碰撞能量, 得到了3-羟基苯并[a]芘的碎片离子, 提高了1-羟基萘、 2-羟基芴、 3-/9-羟基菲和6-羟基屈碎片离子的产率, 并发现活化值Q是电喷雾电离离子阱串联质谱检测不同环数PAHs的关键参数.

关键词: 多环芳烃, 生物标志物, 羟基多环芳烃, 离子阱串联质谱, 碰撞诱导解离

Abstract:

Hydroxylated metabolites of polycyclic aromatic hydrocarbons(OH-PAHs) are the mostly widely used biomarker of carcinogenic PAHs to assess health risk caused by exposure to PAHs. Among present strategies used for detecting OH-PAHs, electrospray ionization tandem mass spectrometry(ESI-MS2) shows advantages in both high sensitivity and selectivity; however, fragment ions cannot be obtained for some OH-PAHs(e.g. 3-OHBaP, 2-OHFlu). In this work, ESI-MS2 analysis of nine OH-PAHs(2- to 5-rings) was achieved with ion trap MS2(IT-MS2); by optimizing collision-induced dissociation conditions of activation Q(AQ) and normalized collision energy(NCE), 3-OHBaP and 2-OHFlu were effectively fragmented meanwhile fragmentation efficiencies of other seven OH-PAHs were also improved. AQ was proven to be the critical parameter for fragmenting OH-PAHs when IT-MS2 is used.

Key words: Polycyclic aromatic hydrocarbon, Biomarker, Hydroxylated metabolite of polycyclic aromatic hydrocarbon, Ion trap tandem mass spectrometry, Collision-induced dissociation

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