高等学校化学学报

高等学校化学学报 ›› 2013, Vol. 34 ›› Issue (1): 123.doi: 10.7503/cjcu20120330

• 生物化学 • 上一篇    下一篇

苯乙酸对肝癌细胞系SMMC-7721的增殖抑制作用及与RNA编辑酶ADAR1表达的相关性

王岩1, 冯子玹2, 姜涛1, 房学东3, 曹宏3, 张研3   

  1. 1. 吉林大学中日联谊医院新民院区普外科, 长春 130033;
    2. 吉林大学计算机科学与技术学院, 长春 130012;
    3. 吉林大学第二医院普外科, 长春 130021
  • 收稿日期:2012-04-10 发布日期:2012-12-31
  • 通讯作者: 王岩,男,主治医师,主要从事肝癌的诱导分化治疗研究.E-mail:wangy0324@gmail.com E-mail:wangy0324@gmail.com
  • 基金资助:

    吉林省科技发展计划项目(批准号: 200705311)资助.

Inhibitory Effects of Phenylacetate on Proliferation and RNA Editing Deaminase ADAR1 Expression of Hepatocellular Carcinoma Cells SMMC-7721

WANG Yan1, FENG Zi-Xuan2, JIANG Tao1, FANG Xue-Dong3, CAO Hong3, ZHANG Yan3   

  1. 1. Department of General Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China;
    2. College of Computer Science and Technology, Jilin University, Changchun 130012, China;
    3. Department of General Surgery, Second Hospital, Jilin University, Changchun 130021, China
  • Received:2012-04-10 Published:2012-12-31

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被引次数

5

摘要：

为探讨苯乙酸(PA)对肝癌细胞系SMMC-7721的增殖抑制作用及其与RNA编辑酶ADAR1表达的相关性, 应用细胞计数及MTT法检测了不同浓度(0.5, 1.0, 2.0和4.0 mmol/L)PA对肝癌细胞系SMMC-7721的增殖抑制作用, 通过流式细胞术(FCM)分析了各细胞周期的细胞百分比, 应用半定量逆转录-聚合酶链式反应(RT-PCR)及免疫印迹杂交分析使用不同浓度(0.5, 1.0, 2.0 mmol/L)PA作用后肝癌细胞系SMMC-7721中RNA编辑酶ADAR1 mRNA及蛋白表达的变化. 结果表明, 肝癌细胞系SMMC-7721经不同浓度PA作用后, 增殖抑制率随作用时间延长及PA浓度增加而明显提高(P<0.05), 但2.0和4.0 mmol/L PA作用72 h后组间差异比较无统计学意义(P>0.05). 肝癌细胞系SMMC-7721中RNA编辑酶ADAR1 mRNA及蛋白表达随PA浓度增加而明显降低(P<0.05). 通过沉默SMMC-7721细胞中ADAR1的表达发现, ADAR1表达下调可有效抑制肝癌细胞增殖. 结果表明, PA可阻抑肝癌细胞系SMMC-7721细胞增殖, 且存在时间及剂量的依赖性, 作用机制与PA下调ADAR1表达相关.

关键词: 苯乙酸, 肝癌, SMMC-7721, RNA编辑酶, ADAR1

Abstract:

To evaluate the inhibitory effects of phenylacetate(PA) on proliferation and RNA editing deaminase ADAR1 expression of hepatocellular carcinoma cells SMMC-7721, the inhibitory effects of different concentrations of PA(0.5, 1.0, 2.0 and 4.0 mmol/L) on hepatocellular carcinoma cells SMMC-7721 were detected by MTT and cell numeration methods. The cell percentages were tested by flow cytometry(FCM). The inhibitory effects of different doses of PA(0.5, 1.0 and 2.0 mmol/L) on the expression of RNA editing dea-minase(ADAR1 mRNA and protein) in SMMC-7721 cells were evaluated by RT-PCR and Western Blotting. With the increasing of concentrations of PA from 0.5 to 4.0 mmol/L and the prolonging time from 24 h to 72 h, the inhibitory rates of proliferation were significantly increased(P<0.05), but there was no significant difference between 2.0 and 4.0 mmol/L PA group for 72 h(P>0.05). With the increasing of concentrations of PA, the expression of RNA editing deaminase(ADAR1 mRNA and protein) was decreased(P<0.05). Further, we made down-regulation of the expression of ADAR1, the results showed that cancer cell proliferation was effectively inhibited. PA could inhibit the proliferation of hepatocellular carcinoma cells SMMC-7721 by the down-regulation of the expression of ADAR1 mRNA and protein in dependence on the time and doses.

Key words: Phenylacetate, Hepatocellular carcinoma, SMMC-7721, RNA editing deaminase, ADAR1

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