Separation and Identification of DABTH-Amino Acids by Reverse-Phase High-Performance Liquid Chromatography

Chem. J. Chinese Universities ›› 1993, Vol. 14 ›› Issue (3): 328.

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Separation and Identification of DABTH-Amino Acids by Reverse-Phase High-Performance Liquid Chromatography

YIN Chang-Chuan, HUANG Wei-Da, SUN Chong-Rong

Department of Biochemistry, Fudan University, Shanghai, 200433

Received:1992-05-09 Revised:2009-09-12 Online:1993-03-24 Published:1993-03-24

Abstract

Abstract: Twenty standard DABTH-amino acids were separated and identified on a reverse-phase high-performance liquid chromatography(RP-HPLC) column with isocratic elution at a sensitivity of picomoles.By using the manual DABTH/PITCdouble-coupling method, we have analyzed and identified the N-terminal amino acid sequence of chicken lysozyme.This technique has been verified to be valuable for protein microsequencing at a level of nanomoles.

Key words: Protein manual microsequencing, DABTH-amino acid, HPLC

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YIN Chang-Chuan, HUANG Wei-Da, SUN Chong-Rong. Separation and Identification of DABTH-Amino Acids by Reverse-Phase High-Performance Liquid Chromatography[J]. Chem. J. Chinese Universities, 1993, 14(3): 328.

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Separation and Identification of DABTH-Amino Acids by Reverse-Phase High-Performance Liquid Chromatography

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