Molecular Recognition of a New Kind of Glycolipid with Concanavallin A in Monolayer Membrane

Chem. J. Chinese Universities ›› 1994, Vol. 15 ›› Issue (5): 783.

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Molecular Recognition of a New Kind of Glycolipid with Concanavallin A in Monolayer Membrane

ZHANG Xi¹, SHEN Jia-Cong¹, Rump E.², Ringsdorf H.²

1. Department of Chemistry, Jilin Unversity, Changchun, 130023;
2. Institute fo Organic Chemistry, University of Mainz, Germany

Received:1993-11-20 Revised:1994-02-24 Online:1994-05-24 Published:1994-05-24

Abstract

Abstract: A new kind of glycolipid bearing p-aminophenyl-α-D-mannopyranoside was synthesized and the direct visual observation of sulforhodamine-labeled concanavallin Abound to the glycolipid monolayer was achieved by using a speciaily designed miniaturized Langmuir trough On the stage of fluorescence microscopy. The glycolipid was spread at a gas phase,and concanavallin Awas injected into the subphase. After incubation for 20 min in 20℃,niany large regular concanavallin Adomains(μ_m level) were observed for the first time. No regular protein domain was found when the dead concanavallin Ainjected,this indicates that the specific interaction between glycolipid and the protein is responsible for the formation of protein domains.

Key words: Glycolipld, Monolayer membrane, Molecular recognition

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ZHANG Xi, SHEN Jia-Cong, Rump E., Ringsdorf H.. Molecular Recognition of a New Kind of Glycolipid with Concanavallin A in Monolayer Membrane[J]. Chem. J. Chinese Universities, 1994, 15(5): 783.

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Molecular Recognition of a New Kind of Glycolipid with Concanavallin A in Monolayer Membrane

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