A Study of Human Albumin Labelled by Silver and Lead with Ion-Selective Electrodes

Chem. J. Chinese Universities ›› 1994, Vol. 15 ›› Issue (9): 1309.

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A Study of Human Albumin Labelled by Silver and Lead with Ion-Selective Electrodes

SHI Sheng-Hua, LIU Peng, HAO Cai-Li, FENG Bin, WANG Yan, LI Wei

Institute of Electroanalytical Chemistry of Chemical Department, Northwest University, Xian, 710069

Received:1993-09-13 Revised:1994-06-12 Online:1994-09-24 Published:1994-09-24

Abstract

Abstract: The complex equilibria of Ag-human albumin (HSA) and Pb-HSAwere studied by ion-selective electrodes.It was clearly proved that both Ag and Pb with HSAformed mononuclear complexes MAand MA2 respectively.The stability products of Ag-HSAcomplexes are β₁₁=4.4×10⁷ and β₁₂=1.0×10₁₂;and that of Pb-HSAcomplexes are β₁₁=3.1×10⁵, β₁₂=2.0×10⁹.According to the immunochemical reaction of HSAwith albumin antiserum (GAHA), the linear range of determing GAHA(titer 1 : 40)is respectively 0.2-2.0μLand 0.4-2.8μLwhen HSAis labelled by Ag and Pb; moreover the linear range of determining HSAis respectively 0-2. 9×10^-8mol/Land 0-7.3×10^-7mol/Lwhen GAHAis labelled by Ag and Pb.

Key words: Ion-selective electrode, Human albumin, Albumin goat antiserum, Complex stability constant, Immunoassay

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SHI Sheng-Hua, LIU Peng, HAO Cai-Li, FENG Bin, WANG Yan, LI Wei. A Study of Human Albumin Labelled by Silver and Lead with Ion-Selective Electrodes[J]. Chem. J. Chinese Universities, 1994, 15(9): 1309.

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A Study of Human Albumin Labelled by Silver and Lead with Ion-Selective Electrodes

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