Catalytic Behavior of Lipase in W/O Microemulsion and Immobilized Lipase in Gelatin Microemulsion-Based Organogels

Abstract

Abstract: The kinetics of the esterification of lauric acid and 1 pentanol catalyzed by Calytical (CL) lipase, was studied in water/bis (2-ethylhexyl)sulfosuccinate sodium(AOT)/isooctane microemulsions. The kinetics studies showed that the reaction followed a Ping Pong Bi-Bi mechanism. The values of all apparent kinetic parameters were determined. The apparent kinetic parameters of the reaction were found to be k_m(lauric acid)=013518 mol/L, k_m(1 pentanol)=022423 mol/Land v_max=13873×10^-5 mol/(L·min·mg). Lipase has also been immobilized in gelatin containing AOT-microemulsion based organogels(MBGs). These lipase containing MBGs prove to be a novel solid-phase catalysts for the use in apolar organic solvents and have a good physical stability. These lipase containing MBGs retained its higher activity after 10 runs esterification reactions.

Key words: W/O microemulsion, Enzyme catalysis, Immobilized enzyme, Microemulsion based gels, Kinetics

CLC Number:

O648.12

TrendMD:

ZHOU Guo-Wei, LI Gan-Zuo, HUANG Xi-Rong, XU Jian, HE Hong-Juan, LI Chuan-Guang . Catalytic Behavior of Lipase in W/O Microemulsion and Immobilized Lipase in Gelatin Microemulsion-Based Organogels[J]. Chem. J. Chinese Universities, 2001, 22(9): 1526.

[1]	SUN Cuihong, LYU Liqiang, LIU Ying, WANG Yan, YANG Jing, ZHANG Shaowen. Mechanism and Kinetics on the Reaction of Isopropyl Nitrate with Cl， OH and NO₃ Radicals [J]. Chem. J. Chinese Universities, 2022, 43(2): 20210591.
[2]	LI Liu, SUN Shiyong, LYU Rui, GOLUBEV Yevgeny Aleksandrovich, WANG Ke, DONG Faqin, DUAN Tao, KOTOVA Olga Borisovna, KOTOVA Elena Leonidovna. Construction of Fe-aminoclay-glucose Oxidase Nanocomposite Catalyst and Its Multi-enzyme Cascade Analysis [J]. Chem. J. Chinese Universities, 2021, 42(3): 803.
[3]	MA Zihui, WANG Mengyan, CAO Hongyu, TANG Qian, WANG Lihao, ZHENG Xuefang. Transient Absorption and Decay Kinetic Properties of Photo-excited Metal Coordinated Tetraphenylporphyrin [J]. Chem. J. Chinese Universities, 2021, 42(3): 767.
[4]	SHUAI Die, ZHAO Meijuan, CHEN Bingnian, WANG Li. Inhibitory Effect of Four Kinds of Keegin-type Phosphomolybdate on Tyrosinase and Melanin Formation and Its Antioxidant Activities [J]. Chem. J. Chinese Universities, 2021, 42(12): 3579.
[5]	ZHANG Aiqin, WANG Man, SHEN Gangyi, JIN Jun. Interactions Between Polybrominated Diphenyl Ethers and Human Serum Albumin Using SPR and Molecular Docking [J]. Chem. J. Chinese Universities, 2020, 41(9): 2054.
[6]	WANG Huan, SUO Jinquan, WANG Chunyan, WANG Runwei. Glucose Oxidase Immobilization with Amino Dendritic Mesoporous Silica Nanoparticles and Its Application in Glucose Detection [J]. Chem. J. Chinese Universities, 2020, 41(8): 1731.
[7]	GAO Xia,PAN Huibin,QIAO Chengfang,CHEN Fengying,ZHOU Yuan,YANG Wenhua. Construction of HRP Immobilized Enzyme Reactor Based on Hierarchically Porous Metal-organic Framework and Its Dye Degradation Application^† [J]. Chem. J. Chinese Universities, 2020, 41(7): 1591.
[8]	WANG Lianping,LI Qingjie,LIU Xiaoyan,REN Yueying,YANG Xiuwei. Screening of Cholinesterase Inhibitors in Fructus Evodiae Alkaloids Based on UFLC-MS/molecular Simulation ^† [J]. Chem. J. Chinese Universities, 2020, 41(1): 111.
[9]	SONG Yichao, HU Mancheng, LI Shuni, ZHAI Quanguo, JIANG Yucheng. Construction of CPO Immobilized Enzyme Reactor Based on Center-radial Dendritic Mesoporous Silica and Its Application ^† [J]. Chem. J. Chinese Universities, 2019, 40(9): 1805.
[10]	SUN Guangdong, PAN Xiaopeng, ZHONG Yuhao, CHEN Enyu, HUANG Yi, SHAO Jianzhong. Photopolymerization Kinetics of Poly(Acrylate Acid) Hydrogels Induced by Blue Light ^† [J]. Chem. J. Chinese Universities, 2019, 40(11): 2404.
[11]	XIAO Biyuan,QIU Jiangyuan,QIN Fanghong,WAN Ting,XU Yaqun,NONG Xiaohui,HUANG Zaiyin. Study on Particle Size Effect on Adsorption Thermodynamics and Kinetics of Cubic Nano-Cu₂O ^† [J]. Chem. J. Chinese Universities, 2019, 40(10): 2214.
[12]	SHI Weiguang,QING Hongxia,ZHANG Shangshang,LU Xiaoyi,WANG Xiaofeng,LI Benxian,XU Xiaohu,LIU Weiguo,WANG Jun,DONG Zhaohui. Kinetics and Abnormal Inhibition of THF on Ethylene Hydrate Formation from Ice Powder Based on Conversion Rate^† [J]. Chem. J. Chinese Universities, 2018, 39(9): 1968.
[13]	REN Huicheng,WU Yingfei,LIU Dandan,NIE Huarong,HE Aihua. Crystallization, Nucleation and Kinetics of TPI in SSBR/TPI Blends^† [J]. Chem. J. Chinese Universities, 2018, 39(5): 1091.
[14]	LUO Juxiang, CHENG Deshu, LI Mingchun, XIN Meihua. Visible Light Induced Rapid Reversible Addition-fragmentation Chain Transfer Polymerization(RAFT )of Styrene at Room Temperature^† [J]. Chem. J. Chinese Universities, 2018, 39(4): 800.
[15]	CHENG Weiliang, ZHU Mengqian, QIN Wu, HOU Cuicui. Chemical Looping Combustion Characteristics of Fe₂O₃(104) and CO Under Synergistic Action of ZrO₂/TiO₂ Carrier^† [J]. Chem. J. Chinese Universities, 2018, 39(3): 506.

Catalytic Behavior of Lipase in W/O Microemulsion and Immobilized Lipase in Gelatin Microemulsion-Based Organogels

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments