Influence of BSA on Cu2+-SCN--H2O2 Chemical Oscillating System

Abstract

Abstract: The influence of bovine serum albumin (BSA) on Cu²⁺-SCN^--H₂O₂ chemical oscillating system was studied. The stable oscillating system contains 0.004 mol/L NaSCN, 0.066×10^-3 mol/L CuSO₄ and 0.111 mol/L H₂O₂, and it was injected during the peak of the third cycle of BSA. The electrode potential-time(E-t) curve was recorded and analyzed. The influence of the concentration of BSA on the chemical oscillating system's cycle prolonging(cycle-slope rate, R_S) can be indicated in a quadratic term equation:
R_S=1.94×10^-3ρ(BSA)²+0.000595ρ(BSA)+0.059292
Protein may affect the chemical oscillation through midbody interference, binding with certain metallic ions, or alteration of solution's physical characteristics, but infraprotein may weaken the physical interference of the chemical oscillation by BSA. A linear relationship between infraprotein of BSA and oscillation cycle is derived with a coefficient correlation of R=0.996, and a linear range of 1—100 mg/L. Therefore, it is a new dynamic quantitative assay for the analysis of protein.

Key words: Chemical oscillation, Bovine serum albumin, Quantitative assay

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SUN Tao¹, GUO Hong-Rui², XU Huan-Lin¹, ZHOU Bao-Kuan¹*. Influence of BSA on Cu²⁺-SCN^--H₂O₂Chemical Oscillating System[J]. Chem. J. Chinese Universities, doi: .

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Influence of BSA on Cu²⁺-SCN^--H₂O₂Chemical Oscillating System

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