Analysis of Vitamin B6 Derivatives in Biological Samples with High Performance Liquid Chromatography

Abstract

Abstract: For the determination of vitamin B₆ derivatives, a high performance liquid chromatography(HPLC) system composed of a single reversed-phase octadecylsilane column and fluorescence detector with an isocratic elution was proposed. This HPLC system perfectly analyzed pyridoxine, pyridoxal, pyridoxamine, pyridoxine 5′-phosphate and pyridoxamine 5′-phosphate together with pyridoxic acid in biological samples. In this method, relatively low fluorescence intensity of pyridoxal 5′-phasphate was improved by converting it to pyridoxic 5′-phosphate with KCN. The analytical procedure is very simple and economical, it is a useful tool for studying vitamin B₆ in animal body.

Key words: High performance liquid chromatography(HPLC), KCN treatment, Fluorescence detection, Vitamin B₆

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ZHANG Jian-Yun, HUANG Long-Quan, HAYAKAWA Takashi, TSUGE Haruhito. Analysis of Vitamin B₆ Derivatives in Biological Samples with High Performance Liquid Chromatography[J]. Chem. J. Chinese Universities, 2004, 25(4): 638.

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Analysis of Vitamin B₆ Derivatives in Biological Samples with High Performance Liquid Chromatography

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