Metabonomics Study on the Acute Toxicity of Toad Venom

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (1): 38.

• Articles • Previous Articles Next Articles

Metabonomics Study on the Acute Toxicity of Toad Venom

LIANG Xiao-Ping^1,2, ZHANG Zheng^1,4, HU Ping³, ZHONG Hong-Fu^1,2, GE Zhao-Hui^1,5, ZOU Ting-Ting^2,6, LIANG Qiong-Lin², WANG Yi-Ming², LUO Guo-An^1,2*

1. School of Phamacy, East China University of Science and Technology, Shanghai 200237, China;
2. Department of Chemistry, Tsinghua University, Beijing 100084, China;
3. College of Chemical and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China;
4. Department of Pharmacology, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;
5. School of Phamacy, Shenyang Pharmaceutical University, Shenyang 110016, China;
6. College of Chemical & Environmental Engineering, Beijing Technology and Business University, Beijing 100048, China

Received:2010-05-31 Revised:2010-10-30 Online:2011-01-10 Published:2010-12-11
Contact: LUO Guo-An E-mail:luoga@tsinghua.edu.cn
Supported by:
国家“新药创制”重大专项基金(批准号: 2008ZX09202, 2009ZX09311-001)和国家“十一五”科技支撑计划项目(批准号: 2006BAI08B04-01)资助.

Abstract

Abstract: In this study, the metabonomics method coupled with electrocardiogram were applied to the study on the acute toxicity of toad venom. The profiles of serum samples were obtained with ultra performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC/TOF-MS). The data were firstly processed with the software Markerlynx and then analyzed with the principal components analysis (PCA) and orthogonal partial least squares discriminate analysis (OPLS-DA). The potential biomarkers were screened out according to the VIP (variable importance in projection) value and identified with i-fit, database and corresponding reference standards. The metabolites of significant intergroup differences were finally found to be lipids related to myocardial energy metabolism. According to the biological functions of the obtained potential biomarkers, it was deduced that the heart injury may be induced by disturbing lipid metabolism through hampering the re-acylation of free fatty acid or activating protein kinase pathway. This study provides a new approach to discover the underlying mechanism of toad venom toxicity.

Key words: Toad venom, Acute toxicity, Metabonomics, UPLC/TOF-MS, Lipid metabolism

TrendMD:

LIANG Xiao-Ping, ZHANG Zheng, HU Ping, ZHONG Hong-Fu, GE Zhao-Hui, ZOU Ting-Ting, LIANG Qiong-Lin, WANG Yi-Ming, LUO Guo-An*. Metabonomics Study on the Acute Toxicity of Toad Venom[J]. Chem. J. Chinese Universities, 2011, 32(1): 38.

[1]	ZHANG Nanxi, LV Jingwei, JIN Ping, LI Jingfeng, BIAN Xuefeng, ZHANG Hui, SUN Jiaming. ¹H NMR Metabonomic Investigations of N-Benzylhexadecanamide Induced Proliferation and Iestosterone Secretion of Mouse Testicular Leydig Cells^† [J]. Chem. J. Chinese Universities, 2019, 40(9): 1832.
[2]	WANG Xiye, SHAN Xiaotong, WANG Yilin, LI Dan, ZHAO Ming, XU Liang. Mechanism of Salvia Miltiorrhiza on the Improvement of Myocardial Function in Patients with Dilated Cardiomyopathy^† [J]. Chem. J. Chinese Universities, 2016, 37(5): 844.
[3]	YANG Yongxia, WANG Linlin, ZHENG Lingyun, WANG Shumei, HUANG Rongbo, ZHANG Lei, HUANG Yaoting. Effects of Huanglian Jiedu Decoction on the Metabolic Features of Brown Adipose Tissue Extracts in Fructose-induced Insulin Resistance Model Based on NMR Metabonomics^† [J]. Chem. J. Chinese Universities, 2014, 35(9): 1883.
[4]	NIU Jun, PI Zi-Feng, YUE Hao, LIU Shu-Ying. Urinary Metabonomics Study of Type 2 Diabetic Rats Treated by Glimepiride [J]. Chem. J. Chinese Universities, 2012, 33(10): 2169.
[5]	LIU Yue-Qin, PI Zi-Feng, SONG Feng-Rui, LIU Zhi-Qiang, LIU Zhong-Ying. Effect of Acarbose on Urine Metabolic Profiling of Type Ⅱ Diabete Rats [J]. Chem. J. Chinese Universities, 2012, 33(09): 1932.
[6]	YANG Ai-Wen, FAN Xue-Mei, LI Xue, LIANG Qiong-Lin, WANG Yi-Ming, LUO Guo-An*. Acute Toxicity of Venenum Bufonis and Compatibility of Heart Musk Protecting Pills by Microarray Expression Analysis [J]. Chem. J. Chinese Universities, 2011, 32(5): 1058.
[7]	LI Ying-Shuai, WANG Qi*, YUAN Zhuo-Jun. NMR-based Metabonomics Studies on Serum and Urine of Yang-deficiency Constitution [J]. Chem. J. Chinese Universities, 2011, 32(11): 2521.
[8]	WANG Wei-Hua, LIU Chun-Hong, SUN Yuan-Ming, LIN Feng, ZHANG Ming-Ming, WANG Zhao-Bin, ZHANG Min-Yi. Metabolic Profiles of Nonylphenol in Rat Urine [J]. Chem. J. Chinese Universities, 2011, 32(10): 2280.
[9]	LIU Xia, XIAO Ying, GAO Hong-Chang, REN Jin, LIN Dong-Hai*. Metabonomic Study of Aristolochic Acid I-induced Acute Renal Toxicity Urine at Female and Male C57BL/6J Mice Based on ¹H NMR [J]. Chem. J. Chinese Universities, 2010, 31(5): 927.
[10]	WEI Yang-Yang, WANG Cai-Hong, LI Wei, DONG Ji-Yang*, CHEN Zhong. NMR-based Metabonomics Study on Serum and Urine of Hyperthyroidism [J]. Chem. J. Chinese Universities, 2010, 31(2): 279.
[11]	HAN Li-Da, LIANG Qiong-Lin, WANG Yi-Ming, LUO Guo-An*. Serum EFAs and NEFAs Metabolic Profiling Analysis Child-bearing Age Women and Its Application to NTDs Preventive Nutrition Intervention [J]. Chem. J. Chinese Universities, 2010, 31(1): 46.
[12]	WANG Yong, JIANG Zhi-Ting, LIANG Qiong-Lin, WANG Yi-Ming, WANG Mei, .... Metabonomics Research of the Influence of Melamine to the Urine Metabolism of the Children Based on UPLC/TOF-MS [J]. Chem. J. Chinese Universities, 2010, 31(1): 57.
[13]	DONG Ji-Yang, XU Le, XU Jing-Jing, CHEN Zhong. Adaptive Binning Method for NMR Spectroscopic Metabonomics Data Preprocessing [J]. Chem. J. Chinese Universities, 2009, 30(6): 1101.
[14]	LIAO Pei-Qiu, WEI Lai, LIU Wei, WU Yi-Jie, LI Xiao-Jing*, NI Jia-Zuan, ....... Metabonomic Studies on Intact Livers and Liver Extracts from Rats Treated with Neodymium Nitrate [J]. Chem. J. Chinese Universities, 2009, 30(6): 1116.
[15]	HUANG Xin, GONG Yi-Fei, WANG Yi*, QU Hai-Bin, CHENG Yi-Yu. Metabonomic Study of Hepato-protective Effect of Silybin for the Carbon Tetrachloride Induced Liver Injure in Mice [J]. Chem. J. Chinese Universities, 2008, 29(4): 714.

Metabonomics Study on the Acute Toxicity of Toad Venom

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments