Preparation of a Functionalized Graphene and Its Role as Delivery Carrier for Anti-cancer Drug†

doi:10.7503/cjcu20160430

Abstract

Abstract:

A novel composite of poly(sodium-p-styrenesulfonate) functionalized graphene nanosheets(PSS-GNS) was prepared through reducing graphene oxide(GO) with hydrazine hydrate as the reducing agent and poly(sodium-p-styrenesulfonate)(PSS) as the protective agent. Then, the properties of PSS-GNS were characterized using ultraviolet-visible spectrometer(UV-Vis), Fourier transform infrared spectrometer(FTIR), X-ray diffraction(XRD), Raman spectrometer, scanning electron microscope(SEM), transmission electron microscope(TEM) and atomic force microscope(AFM). The results reveal that PSS-GNS synthesized are in the form of water-soluble and randomly dispersible nanosheets. Furthermore, the absorption behavior of PSS-GNS for rhodamine 6G used as a model anticancer drug was investigated, which demonstrate that PSS-GNS have a high loading capacity of 2.77 mg/mg. Release profiles in vitro indicate that the release of R6G from PSS-GNS/R6G complexes is pH-dependent and slow-release. PSS-GNS also exhibits low cytotoxicity against cancer cells. PSS-GNS/R6G complexes were easily delivered into the cancer cells and sustained the slow release of R6G for a long time. In conclusion, PSS-GNS could be apromising carrier for anti-cancer drug delivery.

Key words: Functionalized graphene, Anticancer drug delivery carrier, Loading and release, Cytotoxicity

CLC Number:

TrendMD:

LUO Zewei, WANG Yimin, LIU Kunping, WEI Fujing, LI Yu, DUAN Yixiang. Preparation of a Functionalized Graphene and Its Role as Delivery Carrier for Anti-cancer Drug^†[J]. Chem. J. Chinese Universities, 2016, 37(10): 1900.

Figures/Tables 7

Fig.1 FTIR(A) and UV-Vis(B) spectra of PSS(a), GO(b) and PSS-GNS(c)

Fig.2 XRD patterns of natural flake graphite(a) and PSS-GNS(b)(A) and Raman spectra of natural flake graphite(a) and PSS-GNS(b)(B)

Fig.3 SEM(A), TEM(B) and AFM(C) images and height profiles(D) of PSS-GNS

Fig.4 Linear curve of the fluorescence intensity to R6G in different concentrations(A) and fluorescence spectra of R6G loaded on PSS-GNS in different volume(B) (A) The inset describes the corresponding fluorescence spectra of R6G in different concentrations.

Fig.5 Time dependent R6G releasing profiles from PSS-GNS/R6G complexes in pH=7.4(a), pH=4.6(b), pH=2.0(c) buffer(A) and release profiles of R6G from PSS-GNS/R6G complexes in water(a) and ethanol(b) for 2 h(B)

Fig.6 Viability of MDA-MB-231 cancer cells treated with different concentrations of PSS-GNS

Fig.7 Confocal laser scanning microscopy fluorescence images of MDA-MB-231 cancer cells treated with PSS-GNS/R6G for different time The bar is 20.0 μm. (A1—A6) R6G; (B1—B6) Hoechst; (C1—C6) bright field; (D1—D6) merger. Time: (A1—D1) 15 min; (A2—D2) 30 min; (A3—D3) 1 h; (A4—D4) 4 h; (A5—D5) 12 h; (A6—D6) 24 h.

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