PPESO3 Monitoring the Proress of Microwave-enhanced Enzymatic Digestion on Protein

Chem. J. Chinese Universities

• 研究快报 • Previous Articles Next Articles

PPESO₃ Monitoring the Proress of Microwave-enhanced Enzymatic Digestion on Protein

HUAN Yan-Fu¹, ZHANG Zhi-Quan¹, FEI Qiang¹, FENG Guo-Dong^1,2*, MU Ying^2,3*

1. College of Chemistry, Jilin University, Changchun 130012, China;
2. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130021, China;
3. Research Center for Analytical Instrumentation, Institute of Cyber-systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, China

Received:2008-08-21 Revised:1900-01-01 Online:2009-01-10 Published:2009-01-10
Contact: FENG Guo-Dong, MU Ying

Abstract

Abstract: The mini microwave resonance cavity was used to accelerate proteolytic cleavage of protein. A fluorescent polymer PPESO₃ as a fluorescent indicator was used to monitor proteolytic cleavage of protein in the microwave assisted protein digestion process. In this paper, the long-term stability of the PPESO₃ and the PPESO₃ mixed with trypsin was studied, and the detection of proteolytic cleavage of bovine serum albumin(BSA) was obtained satisfactory results. The PPESO₃ was provided a novel, simple, quick determination to set the optimization parameter of the microwave-assisted protein hydrolysis. Meanwhile, the fluorescent detection devices can be integrated into the microwave assisted protein digestion system, for the development of the mini Low-power microwave protein proteolytic system would provide a very good idea.

Key words: Fluorescent conjugated polymer, Microwave-accelerated enzymatic digestion, Protein

CLC Number:

O652.7

TrendMD:

HUAN Yan-Fu¹, ZHANG Zhi-Quan¹, FEI Qiang¹, FENG Guo-Dong^1,2*, MU Ying^2,3*. PPESO₃ Monitoring the Proress of Microwave-enhanced Enzymatic Digestion on Protein[J]. Chem. J. Chinese Universities, doi: .

[1]	ZENG Xianyang, ZHAO Xi, HUANG Xuri. Mechanism of Inhibition of Glucose and Proton Cotransport Protein GlcPSe by Cytochalasin B [J]. Chem. J. Chinese Universities, 2022, 43(4): 20210822.
[2]	CUI Shaoli, ZHANG Weijia, SHAO Xueguang, CAI Wensheng. Revealing the Effect of Threonine on the Binding Ability of Antifreeze Proteins with Ice Crystals by Free-energy Calculations [J]. Chem. J. Chinese Universities, 2022, 43(3): 20210838.
[3]	ZHANG Liling, LIU Liu, ZHENG Mingqiu, FANG Wenkai, LIU Da, TANG Hongwu. Dual Signal Detection of HPV16 DNA by CRISPR/Cas12a Biosensing System Based on Upconversion Luminescent Resonance Energy Transfer [J]. Chem. J. Chinese Universities, 2022, 43(11): 20220412.
[4]	LIU Yunhong, PENG Xinyan. Preparation and Property of A Novel Hemoperfusion Adsorbent For Protein-bound Uremic Toxins [J]. Chem. J. Chinese Universities, 2021, 42(6): 1952.
[5]	LIN Ningqin, YAO Ke, CHEN Xiangjun. Research Progress of Molecular Recognition and Interaction of Crystallins Linking Cataract [J]. Chem. J. Chinese Universities, 2021, 42(11): 3379.
[6]	LIU Xuejiao, YANG Fan, LIU Shuang, ZHANG Chunjuan, LIU Qiaoling. Progress in Aptamer-targeted Membrane Protein Recognition and Functional Regulation [J]. Chem. J. Chinese Universities, 2021, 42(11): 3277.
[7]	CHEN Xiaoyu, LIU Yisheng, HE Mu, SHANGGUAN Ping, HAN Lulu, WANG Jiefei, SHI Bingyang. Recent Advances in Fabricating the Multifunctional Aβ Small Organic Molecule Probes for Theranostic Application [J]. Chem. J. Chinese Universities, 2021, 42(11): 3310.
[8]	YU Lisha, LI Dan, XIAO Liping, FAN Jie. Interaction of Zeolites and Proteins and Its Biomedical Function [J]. Chem. J. Chinese Universities, 2021, 42(1): 311.
[9]	PAN Mengyun, FENG Liuxing, LI Hongmei. Absolute Quantification of Haemoglobin in Whole Blood by Species-unspecific Isotope-dilution Liquid Chromatography-inductively Coupled Plasma Mass Spectrometry [J]. Chem. J. Chinese Universities, 2020, 41(9): 1983.
[10]	LI Huiyuan, LEI Chunyang, HUANG Yan, NIE Zhou. Structural Modification of Fluorescent Proteins and Their Applications in Biosensing [J]. Chem. J. Chinese Universities, 2020, 41(11): 2324.
[11]	LIU Guomin, LU Tiancheng, JI Xuan, JIA Wenyuan, LI Yalong, ZHAO Yian, LUO Yungang. Preparation and Osteogenic Induction Activity of CBD-BMP2-MP/PLGA 3D Printed Composite Scaffolds^† [J]. Chem. J. Chinese Universities, 2019, 40(7): 1552.
[12]	WEI Wanghui,CHU Yanqiu,CHEN Ying,Gao Yanqiu,DING Chuanfan. Quantitative Determination of the Glycosylation Level for the Binding Proteins to High Density Lipoprotein by Mass Spectrometry^† [J]. Chem. J. Chinese Universities, 2019, 40(6): 1141.
[13]	ZHANG Chenglu,GONG Rongqing,YANG Jingyi,SUN Xiaona,LI Yilin,WANG Huayu,SONG Fulu,SUN Yuedong. Synthesis of Novel Polyheterocyclic Molecules with 1,3,4-Oxadiazole Thioetheramide as Core Framework and Their Inhibitory Activity on Cdc25B and PTP1B^† [J]. Chem. J. Chinese Universities, 2019, 40(2): 262.
[14]	ZHANG Yu, JING Jiangbo, SHAO Yueming, YIN Xin, XU Bin, WEN Xiaoyu. Specific Hepatocellular Carcinoma Imaging Based on Aggregation-Induced Emission Nanoparticles ^† [J]. Chem. J. Chinese Universities, 2019, 40(11): 2382.
[15]	LIU Zhongcheng, LIU Shifang, ZHANG Su, YANG Yanlei, LI Fei, ZHANG Nan, YUAN Xin, ZHANG Yanfen. Structure Prediction and Screening of Oligonucleotide Aptamers Target Cε3-Cε4 Protein^† [J]. Chem. J. Chinese Universities, 2019, 40(1): 83.

PPESO₃ Monitoring the Proress of Microwave-enhanced Enzymatic Digestion on Protein

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments