Preparation and Characterization of Chitosan Derivative Microspheres for Protein Delivery†

doi:10.7503/cjcu20140665

Abstract

Abstract:

A novel amphiphilic narrow molecular weight distribution and low degree of polymerization lauryl-succinyl chitosan derivative(LSCOS) was synthesized in ionic liquid homogeneous system. The LSCOS microspheres were prepared according to water-in-oil emulsification cross-linking method with LSCOS as protein-loading material and bovine serum albumin(BSA) as a model protein and glutaraldehyde as the crosslinker. The effects of the BSA/LSCOS mass ratio and glutaraldehyde/LSCOS mass ratio on the morphology structure, loading capacity(LC), loading efficiency(LE) and in vitro BSA release characteristics were investigated by means of scanning electron microscopy(SEM), transmission electron microscopy(TEM) and UV-Vis spectroscopy. The results showed that LSCOS was successfully synthesized in ionic liquid and BSA-loaded LSCOS microspheres were spherical in shape with particle size approximately 1 μm. The microspheres had a smoother surface with the increasing ratio of BSA/LSCOS and a rougher surface with the increasing ratio of glutaraldehyde/LSCOS. Decrease and increase in the ratio of BSA/LSCOS and glutaraldehyde/LSCOS, respectively, resulted in the decrease of the cumulative release of BSA. The data indicated that protein release rate can be controlled by adjusting the protein ratio and the glutaraldehyde ratio. Therefore, the LSCOS synthesized in ionic liquid could be a promising material with slow-release function for protein delivery.

Key words: Ionic liquid, Chitosan, Derivative, Emulsification cross-linking method, Microsphere

CLC Number:

O636.1

TrendMD:

LIU Yuanyuan, HUANG Yan, LU Zhengrong, BOAMAH Peter-Osei, ZHANG Qi, WU Jingbo, HUA Mingqing. Preparation and Characterization of Chitosan Derivative Microspheres for Protein Delivery^†[J]. Chem. J. Chinese Universities, 2015, 36(3): 589.

Figures/Tables 9

Scheme 1 Synthetic routes of LSCOS

Fig.1 1H NMR spectra of COS(a), SCOS(b) and LSCOS(c)

Fig.2 FTIR spectra of COS(a), SCOS(b) and LSCOS(c)

Fig.3 SEC spectra of standard samples with various weight-average molar mass(A) and COS(B,a),SCOS(B,b) and LSCOS(B,c) The data indicated were the retention time(t) and the weight-average molar mass(Mˉ).

Fig.4 SEM images of BSA/LSCOS microspheres with different m(BSA):m(LSCOS) m(BSA):m(LSCOS): (A) 0:100; (B) 25:100; (C) 50:100. Inset of (A): SEM image of froctured microspheres of (A).

Fig.5 SEM images of BSA/LSCOS(50:100) microspheres cross-linked with m(glutaraldehyde):m(LSCOS) of 5:100(A, B) and 10:100(C, D)

Fig.6 TEM images of LSCOS microspheres(A) for blank control and BSA/LSCOS(50:100, B) microspheres

Table 1 LC and LE of BSA/LSCOS microspheres

m(BSA):m(LSCOS)	m(Glutaraldehyde):m(LSCOS)	LC(%)	LE(%)
25:100		8.1	42
50:100		17.0	52
50:100	5:100	18.1	56
50:100	10:100	19.6	59

Fig.7 BSA accumulative release curves of non-cross-linked(a, b) and cross-linked BSA/LSCOS microspheres a. m(BSA):m(LSCOS)=25:100; b. m(BSA):m(LSCOS)=50:100; c. m(BSA):m(LSCOS)=50:100, m(Glutaraldehyde):m(LSCOS)=5:10; d. m(BSA):m(LSCOS)=50:100, m(Glutaraldehyde):m(LSCOS)=10:100.

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