Effect of Carbon Sources on the Performance of Methane-producing Biocathodes†

doi:10.7503/cjcu20140727

Abstract

Abstract:

Effects of organic carbon source(OCS) and inorganic carbon source(ICS) on the performance of methane-producing biocathodes were studied. The current density, methanation, polarization curves and biomass attached were investigated during both start-up and stable operating periods. The results show that OCS not only accelerated formation of the methane-producing biocathode, but improved its operating performance. As for the polarization tests, the current density of the OCS-associated biocathode could reach (2.34±0.15) A/m² with the cathode potential poised at -0.75 V(vs. SHE), higher than that of the ICS-associated bioca-thode. Compared to ICS addition, OCS addition could achieve in-situ supply of carbon dioxide(CO₂), which would alleviate gas-liquid transfer limitations and promote microorganisms’ growth. In this case, the biomass attached on OCS-associated biocathode was more than 4 times as much as that on ICS-associated biocathode, further elucidating the high-performance of OCS-associated methane-producing biocathodes.

Key words: Organic carbon source(OCS), Inorganic carbon source(ICS), Methane-producing biocathode, Current density, Biomass

CLC Number:

O646.5

TrendMD:

XU Heng, WANG Cuiping, WANG Kaijun. Effect of Carbon Sources on the Performance of Methane-producing Biocathodes^†[J]. Chem. J. Chinese Universities, 2015, 36(2): 344.

Figures/Tables 7

Fig.1 H-cell used in the experiment

Table 1 Operational parameters adopted in the experiment

Stage	Addition of glucose stock solution/mL	Medium replacement time/d	Medium replacement ratio(%)
Startup period(0—14 d)	0.2	3rd, 6th, 12th	20
Experimental period(15—24 d)	0.2	17th, 22nd	40
Experimental period(25—33 d)	0.4	28th	40

Fig.2 Startup of methane-producing biocathodes with glucose(OS, a) and CO2(IS, b) as carbon sources, respectively MR: medium replacement.

Fig.3 Current density of methane-producing biocathodes during the stable operation period MR: medium replacement. a. OS; b. IS.

Fig.4 Volume and CH4 content of biogas produced during the stable operation period

Fig.5 Polarization curves of methane-producing biocathodes with glucose(OS, a) and CO2(IS, b) as carbon source, respectively

Fig.6 Total protein recovered from methane-producing biocathodes and their surrounding liquid with glucose(OS) and CO2(IS) as carbon sources, respectively

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