Preparation and Chromatographic Behavior of Novel Tetrazole Bonded Stationary Phase for Ion-exchange Chromatography of Proteins

Chem. J. Chinese Universities

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Preparation and Chromatographic Behavior of Novel Tetrazole Bonded Stationary Phase for Ion-exchange Chromatography of Proteins

LEI Gen-Hu¹, XIONG Xiao-Hu¹, HUO Yan-Min¹, WEI Yin-Mao^1,2*, ZHENG Xiao-Hui²

1. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Department of Chemistry,
2. Research and Engineering Center of Modernization of Chinese Medicine, Northwest University, Xi′an 710069, China

Received:2007-06-26 Revised:1900-01-01 Online:2008-02-10 Published:2008-02-10
Contact: WEI Yin-Mao

Abstract

Abstract: A novel tetrazole bonded ion-exchange stationary phase was presented. Tetrazole-functionalized stationary phase was prepared by treatment of silica gel with γ-glycidoxypropyltriethoxysilane, then 3-hydroxypropionitrile, followed by the Zn(Ⅱ)-catalyzed(3+2) azide-nitrile cycloaddition, which is an element of “click chemistry”. The resulting column(4.6 mm×50 mm i.d.) exhibited an excellent separation ability for proteins with high protein mass recoveries of more than 93%, and displayed a property of weak cation ion-exchange chromatography, but the changes in the retentions of protein with pH variation were diffe-rent from those obtained on the carboxylic acid bonded ion-exchanger, for which the reason was preliminarily explained.

Key words: Ion-exchange chromatography, Stationary phase, Ligand, Tetrazole, Biopolymers

CLC Number:

O657

TrendMD:

LEI Gen-Hu¹, XIONG Xiao-Hu¹, HUO Yan-Min¹, WEI Yin-Mao^1,2*, ZHENG Xiao-Hui². Preparation and Chromatographic Behavior of Novel Tetrazole Bonded Stationary Phase for Ion-exchange Chromatography of Proteins[J]. Chem. J. Chinese Universities, doi: .

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Preparation and Chromatographic Behavior of Novel Tetrazole Bonded Stationary Phase for Ion-exchange Chromatography of Proteins

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