Preparation of Organicinorganic Nanoparticles Composite and Their Laccase Immobilization

Chem. J. Chinese Universities

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Preparation of Organicinorganic Nanoparticles Composite and Their Laccase Immobilization

HUANG Jun, WANG Jun-Tao, LI Bin, XIAO Hai-Yan, LIU Cheng, DING Li-Yun, JIANG De-Sheng

Key Laboratory of Fiber Optic Sensing Technology and Information Processing, Ministry of Education, Fiber Optic Sensing Technology Research Center, Wuhan University of Technology, Wuhan 430070, China

Received:2005-11-07 Revised:1900-01-01 Online:2006-11-10 Published:2006-11-10
Contact: HUANG Jun

Abstract

Abstract: CoTAPc-Fe₃O₄ nanoparticle composites were prepared by organic-inorganic nanoparticles complex technology and characterized by IR, XRD, EDS, field emission gun scanning electron microscopy and magnetometer. It is proved that the CoTAPc could disperse randomly onto the surface of Fe₃O₄ nanoparticles. The nanoparticles composites had the shape of spheres with the mean diameter about 70 nm. They had the coercive force of 316.4 A/m and were approximately superparamagnetic. The laccase was immobilized on the surface of the composite by crosslinking method. The optimal temperature and pH forthe laccase immobilization were 45 ℃ and pH 3.0, respectively. The immobilized laccase had better thermal, storage and operation stabilities than free laccase. This work provide a good basis for developing the fiber optic biosensor with excellent properties.

Key words: CoTAPc-Fe₃O₄ nanoparticles composite, Lac case, Immobilization

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HUANG Jun, WANG Jun-Tao, LI Bin, XIAO Hai-Yan, LIU Cheng, DING Li-Yun, JIANG De-Sheng. Preparation of Organicinorganic Nanoparticles Composite and Their Laccase Immobilization[J]. Chem. J. Chinese Universities, doi: .

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Preparation of Organicinorganic Nanoparticles Composite and Their Laccase Immobilization

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