Mercaptoacetic Acid Self-assembled DNA Electrochemical Biosensor for Detection of NOS Gene Sequence

Chem. J. Chinese Universities

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Mercaptoacetic Acid Self-assembled DNA Electrochemical Biosensor for Detection of NOS Gene Sequence

SUN Wei, SHANG Zhi-Mei, YANG Mao-Xia, JIAO Kui

College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China

Received:2006-01-09 Revised:1900-01-01 Online:2006-10-10 Published:2006-10-10
Contact: SUN Wei

Abstract

Abstract: A NOS gene electrochemical biosensor was prepared by covalently mmobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride(EDC) and N-hydroxysuccinimide(NHS). By using methylene blue as the electrochemical indicator, the increase of cathodic peak current was in linear with the concentration of complementary ssDNA in the concentration range of 5.0×10^-8—1.0×10^-4 mol/L with the linear regression equation as ΔI_pc=1.10lgc+8.25(n=8, γ=0.998 5) and the detection limit as 3.60×10^-8mol/L(3σ).

Key words: Electrochemical DNA biosensor, Mercaptoacetic acid, NOS gene, Methylene blue, Self-assembled film electrode

CLC Number:

O657

TrendMD:

SUN Wei, SHANG Zhi-Mei, YANG Mao-Xia, JIAO Kui. Mercaptoacetic Acid Self-assembled DNA Electrochemical Biosensor for Detection of NOS Gene Sequence[J]. Chem. J. Chinese Universities, doi: .

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Mercaptoacetic Acid Self-assembled DNA Electrochemical Biosensor for Detection of NOS Gene Sequence

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