Simultaneous Extraction of Salidroside and Tyrosol from Rhodiolarosea L. Using Tailor-made Deep Eutectic Solvents†

doi:10.7503/cjcu20180669

Abstract

Abstract:

A method for simultaneously extracting the salidroside and tyrosol from Rhodiolarosea L. using deep eutectic solvents(DESs) as the extraction solvents was developed in this work. Firstly, the hydrogen bond donor(HBD), hydrogen bond acceptor(HBA), molar ratio of HBA to HBD, and water content were optimized. The optimal DES for the simultaneous extraction was obtained as ethylene glycol-levulinic acid at the molar ratio of 1∶1 and water content of mass fraction of 40%, which was recorded as LAEG-40. Then, taking the LAEG-40 as the extraction solvent, the extraction conditions such as extraction method, solid to solvent ratio, extraction temperature and extraction time were further optimized. The optimal conditions were obtained as follows: stirring extraction at the solid to solvent ratio of 1∶12.5(g/mL) and 60 ℃ for 65 min. Under these conditions, the extraction yields of salidroside and tyrosol reached (18.1268±0.1667) and (1.5608±0.0240) mg/g, respectively. However, under the same conditions, taking water or ethanol as the extraction solvents, the extraction yields of salidroside and tyrosol were (15.1221±0.1342) and (1.1120±0.0389) mg/g, or (16.3425±0.0897) and (1.1923±0.0423) mg/g, respectively. It could be seen that the extraction efficiency of LAEG-40 was obviously higher than those of traditional solvents. The results showed that LAEG-40 was a green and efficient solvent for simultaneous extraction of salidroside and tyrosol, which could be used as a substitute for traditional solvents.

Key words: Salidroside, Tyrosol, Deep eutectic solvent, Green solvent, Simultaneous extraction

CLC Number:

O629

TrendMD:

WU Rong,WANG Huimin,CAO Jun,CHEN Luyao,SU Erzheng. Simultaneous Extraction of Salidroside and Tyrosol from Rhodiolarosea L. Using Tailor-made Deep Eutectic Solvents^†[J]. Chem. J. Chinese Universities, 2019, 40(5): 918.

Figures/Tables 10

Scheme 1 Simultaneous extraction of salidroside and tyrosol from Rhodiolarosea L. using deep eutectic solvents

Fig.1 Extraction yields of the choline chloride based DESs for salidroside(A) and tyrosol(B)a—t: DES-1; DES-2; DES-4; DES-5; DES-7; DES-8; DES-9; DES-10; DES-11; DES-12; DES-14; DES-15; DES-16; DES-17; DES-18; DES-19; DES-20; DES-21; DES-24; DES-26.

Fig.2 Extraction yields of the betaine based DESs for salidroside(A) and tyrosol(B) a. DES-28; b. DES-31; c. DES-32; d. DES-33; e. DES-34; f. DES-37; g. DES-38.

Fig.3 Extraction yields of the DESs formed between different organic acids and lcohols for salidroside(A) and tyrosol(B)a—i: DES-42; DES-43; DES-44; DES-45; DES-46; DES-47; DES-48; DES-49; DES-50.

Fig.4 Extraction yields of several DESs with different component molar ratios for salidroside(A) and tyrosol(B)

Fig.5 Extraction efficiency of different extraction methods using LAEG-40 as the extraction solvent at 25 and 60 ℃A, D. Water bath shaking extraction; B, E. air bath shaking extraction; C, F. stirring extraction; G. heat extraction. A—C. 35 ℃; D—G. 60 ℃.Solid/solvent ratio: 1∶10(g/mL), extraction time: 5 min.

Fig.6 Effect of solid/solvent ratio on the extraction yields of salidroside and tyrosol air-bath shaking(A) and stirring(B) at 150 r/min and 60 ℃ for 5 min, respectively

Fig.7 Effect of extraction temperature on the extraction yields of salidroside and tyrosol

Fig.8 Effect of extraction time on the extraction yields of salidroside and tyrosol

Table 1 Comparison of the extraction efficiency of LAEG-40 with the conventional solvents under the optimized extraction conditions

Solvent	Salidroside extraction efficiency/(mg·g^-1)	Tyrosol extraction efficiency/(mg·g^-1)
LAEG-40	18.0879±0.2664	1.6208±0.0471
Water	15.1221±0.1342	1.1120±0.0389
Ethanol	16.3425±0.0897	1.1923±0.0423

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