Abstract: The differentially expressed genes involved in acute toxicity and compatibility of Heart Musk Protecting Pills(HMP) via cDNA microarray were investigated in this paper. Then the GO and KEGG Pathway enrichment analysis were carried as well as the functional analysis and real time RT-PCR validation of two important genes. Results indicated that the ion homeostasis and actin organization were affected in the low dosage group for Venenum Bufonis, Meanwhile, regulation of anti-apoptosis and fatty acid metabolism could be induced within two hours; the myocardial contraction was finally disturbed. The ion homeostasis and actin organization could also be disturbed more quickly in the high dosage group. In addition, the iron accumulation could lead to oxidation reduction and cardiomyocyte apoptosis. For HMP, there were some clinical functions, such as blood pressure regulation and myocardial structure organization. However, the related acute toxicity of Venenum Bufonis was not shown. This paper could provide a novel research strategy in the compatibility study of Traditional Chinese Medicine (TCM).

Key words: Microarray, Real time RT-PCR, Venenum Bufonis, Heart Musk Protecting Pills(HMP)；Acute toxicity；Compatibility of Traditional Chinese Medicine(TCM)