CJCU

Chem. J. Chinese Universities ›› 2013, Vol. 34 ›› Issue (5): 1078.doi: 10.7503/cjcu20130029

• Analytical Chemistry • Previous Articles     Next Articles

Detecting Type 2 Diabetes-related Single Nucleotide Polymorphisms by Microarray

CHEN Yan1,4, LIU Gui-Feng2, LIU Xia3, ZHANG Gui-Zhen1, WANG Zhen-Xin3   

  1. 1. Central Research Department, China-Japan Union Hospital of Jilin University, Changchun 130033, China;
    2. Radiology Department, China-Japan Union Hospital of Jilin University, Changchun 130033, China;
    3. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, China;
    4. Endocrinology Department, The Second Hospital of Jilin University, Changchun 130042, China
  • Received:2013-01-08 Online:2013-05-10 Published:2013-05-10
  • Contact: CHEN Yan E-mail:zhangguizhenjl@yahoo.com.cn

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Abstract:

A three-dimensional(3D) oligonucleotide microarray-based fluorescent assay was developed for screening specific oligonucleotide probes for type 2 diabetes detection. The 3D oligonucleotide microarray was fabricated on the home-made fourth-generation(G4) NH2-terminated poly(amidoamine) dendrimers(PAMAM) modified substrate. Assay for rs7903146 site of the type 2 diabetes related gene TCF7L2 was developed. The utility of this assay was demonstrated with an oligonucleotide library, a collection of 16(8 pairs) oligonucleotides, and one specific oligonucleotide probe pair for type 2 diabetes was obtained. Taking advantage of the high specificity of the probe pair, the allele frequency as low as 2% can be accurately determined.

Key words: Oligonucleotide microarray, Type 2 diabetes, Allele frequency, Oligonucleotide probe

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