CJCU

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (4): 679.

• Articles • Previous Articles     Next Articles

Isolation and Purification of a New Shikimic Acid Derivative from Schisandra Chinensis and Its Content Assay

ZHU Hong-Yan1, MENG Xiang-Ying1*, BAO Yong-Li2,3, YU Chun-Lei2,3, WU Yin2,3, LI Yu-Xin2,3*   

  1. 1. Institute of Genetic and Cytology,
    2. Research Center of Agriculture and Medicinal Gene Engineering of Ministry of Education,
    3. National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun 130024, China
  • Received:2009-09-30 Online:2010-04-10 Published:2010-04-10
  • Contact: MENG Xiang-Ying. E-mail: mengxy377@nenu.edu.cn; LI Yu-Xin. E-mail: liyx486@nenu.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 30670220)、教育部重点项目(批准号: 107121)、教育部新世纪优秀人才项目(批准号: NCET-060320)、吉林省科技发展计划基金(批准号: 20060904)及北师范大学创新科技项目培育基金(批准号: NENU-STB07008)资助.

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Abstract:

A new shikimic acid derivative(1) was isolated from the ethanol extract of Schisandra chinensis(Turcz.) Baill. The structure of compound 1 was elucidated as n-butyl shikimate, on the basis of the 1D NMR, HMQC and HMBC spectroscopic techniques. A RP-HPLC method was performed in a extend C18 column with a gradient elution mobile phase of acetonitrile-0.05%H3PO4 water solution at a flow rate of 1.0 mL/min, and the detection wavelength was 215 nm. A good linearity for n-butyl shikimate was obtained within the concentration range of 2—65 μg/mL(r=0.9999), and the recovery was 98.25% with RSD 1.55%. The method was simple, accurate with good repeatability.

Key words: Schisandra chinensis(Turcz.) Baill.; n-Butyl shikimate; High performance liquid chromatography(HPLC)

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