CJCU

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (4): 684.

• Articles • Previous Articles     Next Articles

Gene Expression Studies of Melamine-related Renal Toxicity Based on cDNA Microarray

SHI Mei1, FAN Xue-Mei1, LI Xue2* , WANG Zheng3, WANG Yi-Ming2, LUO Guo-An1,2*   

  1. 1. School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China;
    2. Modern Research Center of TCM, Tsinghua University, Beijing 100084, China;
    3. School of Pharmacy, Nankai Universities, Tianjin 300071, China
  • Received:2009-07-22 Online:2010-04-10 Published:2010-04-10
  • Contact: LUO Guo-An. E-mail: luoga@tsinghua.edu.cn; LI Xue. E-mail: lixue@mail.tsinghua.edu.cn
  • Supported by:

    国家“九七三”计划项目(批准号: 2007CB714505)、国家“十一五”科技支撑项目(批准号: 2006BAI08BO4-01)和教育部博士点基金(批准号: 20080031012)资助.

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Abstract:

Nowadays, microarray technology has been used in toxicogenomics more and more often. In this paper, we applied it into the renal toxicity research of melamine, which is an intermediate of resin in chemical industry. The experimental, through microarray and bioinformatics, was designed to find the differences between two cases in which the rats independently intervened by high and low doses melamine, and then to interpret the molecular mechanism of renal toxicity. The result indicated that the number of differentially expressed genes intervened by high dose of melamine were more than that by low dose of melamine. Besides, the differential expressed genes interfered by high dose melamine have been proved to have close connection with some important pathways as follow: valine, leucine and isoleucine degradation, citrate metabolism, glutathione metabolism, fatty acid metabolism, and the molecular function such as mental binding, lyase activity, ligase activity, symporter activity, glutathione transferase activity, monooxygenase activity. Our research show the toxicity in kidney intervened by melamine, and demonstrated the special advantage of microarray technology in toxicogenomics.

Key words: Melamine; Microarray; Nephrotoxicity; Nephrolithiasis

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