Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (9): 1763.

• Articles • Previous Articles     Next Articles

Proteome Study of Differential Protein Expression in HBV-related Primary Hepatic Carcinoma

LIU Chi-Bo1, LIANG Yong1,2*, PAN Chun-Qin1, WANG Hai-Bao1, MOU Si-Hua1   

  1. 1. Taizhou Municipal Hospital, Affiliated to Medical College of Taizhou University,
    2. Medical College of Taizhou University, Taizhou 318000, China
  • Received:2009-01-17 Online:2009-09-10 Published:2009-09-10
  • Contact: LIANG Yong. E-mail: langy@yahoo.cn
  • Supported by:

    国家“八六三”计划(批准号: 2006AA02090406B)资助.

Abstract:

To detect and identify differentially expressed proteins in the sera of patients with HBV-related primary hepatic carcinoma. The surface-enhanced laser desorption/ionization time-of-flight mass spectroscopy(SELDI-TOF-MS) was used to screen for the differentially expressed serum proteins in patients with HBV-related primary hepatic carcinoma and healthy adults. CM10(weak cation exchange)chip found 44 differentially expressed protein peaks(P<0.05) in HBV-related primary hepatic carcinoma group compared with those in the healthy adult group, 21 proteins in patients with HBV-related primary hepatic carcinoma are up-regulated, 23 proteins are down-regulated. Then the most differential protein sample was isolated and purified by high perfomance liquid chromatographic and was subjected to mass spectrometry analysis. They were listed as follows: Serum amyloid A and Serum amyloid A1. Identified in the study could be associated with the pathogenesis of HBV-related primary hepatic carcinoma.

Key words: HBV-related primary hepatic carcinoma; Surface-enhanced laser desorption/ionization time-of-flight mass spectroscopy; Proteomics

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