Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (8): 1571.

• Articles • Previous Articles     Next Articles

Alcohol-induced Conformation of Myoglobin

JIANG Dan, CHU Yan-Qiu, DING Chuan-Fan*   

  1. Laser Chemistry Institute, Department of Chemistry, Fudan University, Shanghai 200433, China
  • Received:2008-12-18 Online:2009-08-10 Published:2009-08-10
  • Contact: DING Chuan-Fan. E-mail: cfding@fudan.ac.cn
  • Supported by:

    国家自然科学基金(批准号: 25027004)资助.

Abstract:

This paper mainly reports the alcohol-induced effection. Accoding to the mass spectrometry, we got a lot differences among myoglobin conformations induced by different alcohol at pH=4.0, 30%(volume fraction) alcohol. The results show that alcohol′s molecular structures effect Mb conformation. Besides, we found that the alkyl′s number enhanced denaturation effection. Fluorine substituent could stabilize hMb and aMb selectively a certain extent. we also proved the regularity summarized as above farther and got more useful information of Fe ion′s coordination and election spin state via CD spectroscopy and UV-Vis Soret Absorption.

Key words: Protein; Molecular conformation; Myoglobin; Alcohol-induced

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