Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (8): 1538.

• Articles • Previous Articles     Next Articles

Real-time Monitoring Continuous Replication of DNA Leading Strand in vitro Based on Molecular Beacon

MENG Xiang-Xian, YANG Xiao-Hai, WANG Ke-Min*, GUO Qiu-Ping, LI Jun, TANG Zhi-Wen   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Biomedical Engineering Center, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China)

  • Received:2009-03-10 Online:2009-08-10 Published:2009-08-10
  • Contact: WANG Ke-Min. E-mail: kmwang@hnu.cn
  • Supported by:

    国家自然科学基金(批准号: 20505007, 90606003)和湖南省自然科学基金(批准号: 08JJ1002) 资助.

Abstract:

Real-time monitoring of replication of DNA leading strand using molecular beacon in vitro was reported. DNA replication plays a pivotal role in life process. DNA replication is usually assayed by denatured gel electrophoresis and autoradiography, which are complex, time-consuming, and incapable of acquiring the dynamic data in real time. We employed molecular beacon as the probe and template for DNA replication and converted directly replication information into fluorescence signal. The replication information of DNA leading strand was obtained conveniently and accurately using molecular beacon. This method would provide a strategy for real-time monitoring DNA replication and for further research of replication dynamic data and some deseases associated with DNA replication.

Key words: Molecular beacon; DNA replication; Real-time monitoring; Fluorescent signal

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