Study on Role for Propecting HUVEC Damaged by Urate and Content Variation of Alkaloids Components in Ermiao Wan Categorized Formula

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (8): 1522.

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Study on Role for Propecting HUVEC Damaged by Urate and Content Variation of Alkaloids Components in Ermiao Wan Categorized Formula

YIN Lian^1,2*, DENG Hai-Shan¹, ZHU Xiao-Qin¹, LI Xin¹, DUAN Jin-Ao², DING An-Wei¹

1. Pharmacological College,
2. Key Laboratory for Modern Research of Traditional Chinese Medical Formula of Jiangsu, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China

Received:2008-11-28 Online:2009-08-10 Published:2009-08-10
Contact: YIN Lian. E-mail: Yinlian162@163.com
Supported by:
江苏省自然科学基金(批准号: BK2002033)、江苏省方剂研究重点实验室开放项目(批准号: 022021014010)和南京中医药大学基础研究与重点培育项目(批准号: 08XPY04)资助.

Abstract

Abstract:

To reveal the compatibility of traditional Chinese medicine, the material foundation playing major role of Ermiao Wan categorized formula and their compatibility content variation was studied. The activity of the combined effective components by orthogonal design in its relevant herbs were evaluated for protecting HUVEC damaged model induced by urate, the results indicated that the alkaloids components of Phellodendron chinense(PC) of Ermiao Wan play a major role in its categorized formula. The fingerprint of alkaloids components in PC and Ermiao Wan categorized formula were analyzed and compared by high performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-ESI-MS). Twelve alkaloids were detected in the fingerprint of alkaloids. The contents of some alkaloids were found to be reduced, on the contrary, the contents of total alkaloids were increased 122.9% in Ermiao Wan, and reduced 80.7%, 60.2%, 94.2% in its categorized formula. From the chemical point of view, the material foundation of basic formula plays major role in its categorized formula, the variation of relative content of alkaloids are in accord with the virtue of Ermiao Wan categorized formula.

Key words: Ermiao Wan categorized formula; Alkaloid; Fingerprint; Compatibility; HPLC-ESI-MS/MS

TrendMD:

YIN Lian*, DENG Hai-Shan, ZHU Xiao-Qin, LI Xin, DUAN Jin-Ao, DING An-Wei. Study on Role for Propecting HUVEC Damaged by Urate and Content Variation of Alkaloids Components in Ermiao Wan Categorized Formula[J]. Chem. J. Chinese Universities, 2009, 30(8): 1522.

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Study on Role for Propecting HUVEC Damaged by Urate and Content Variation of Alkaloids Components in Ermiao Wan Categorized Formula

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