Photosynthesis and Differentially Expressed Proteins in Two Morphologic Filaments of Spirulina platensis

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (6): 1152.

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Photosynthesis and Differentially Expressed Proteins in Two Morphologic Filaments of Spirulina platensis

LIN Chong-Yang, SHEN Xiao-Wen, ZHAO Zhen-Kun, WU Han-Zhi, XU Hong*

The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, China

Received:2008-08-11 Online:2009-06-10 Published:2009-06-10
Contact: XU Hong, E-mail: hxu@xmu.edu.cn
Supported by:
国家自然科学基金(批准号: 30571446)和福建省高等学校新世纪人才支持计划资助.

Abstract

Abstract:

The two kinds of different morphological filaments(spiral and linear ) of Spirulina platensis were separated and grown as axenic cultures. The photosynthetic characteristics show that compared with linear filaments, spiral filaments have higher light-saturated photosynthetic rate and light saturation point, and appeare to be more acclimated to high light intensity. Whereas linear filaments might be more acclimated to low light intensity for their lower light compensation point. To explore the morphologensis determination mechanism of Spirulina, the proteins that were differentially expressed in the spiral and the linear forms were studied. Two dimensional gel electrophoresis was performed to separate differentially expressed proteins that were subsequently identified by MALDI-TOF mass spectrometry. The 2-DE and PMF analysis revealed that at least nine proteins or subunits might relate to the morphogenesis of Spirulina platensis. The identified proteins include three proteins related to peptidoglycan metabolism, two photosynthetic proteins, one transmembrane channel protein, one protein related to cell division, as well as two unknown proteins.

Key words: Spirulina platensis, Morphogical transformation, Photosynthesis, Two-dimensional gel electrophoresis, Differentially expressed protein

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LIN Chong-Yang, SHEN Xiao-Wen, ZHAO Zhen-Kun, WU Han-Zhi, XU Hong*. Photosynthesis and Differentially Expressed Proteins in Two Morphologic Filaments of Spirulina platensis[J]. Chem. J. Chinese Universities, 2009, 30(6): 1152.

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Photosynthesis and Differentially Expressed Proteins in Two Morphologic Filaments of Spirulina platensis

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