Abstract:

SiO₂-CaO-P₂O₅ gel bioglass(BG) nanoparticles with the diameter of 40 nm were synthesized by sol-gel approach. The surface of BG nanoparticles was grafted through the ring-open polymerization of the L-lactide to yield poly(L-lactide)(PLLA) grafted gel particle(PLLA-g-BG). The PLLA-g-BG was further blended with poly(lactide-co-glycolide)(PLGA) to prepare the nanocomposites of PLLA-g-BG/PLGA with the various blend ratios of two phases. PLLA-g-BG accounted 10%, 20% and 40% in the composite, respectively. TGA, ESEM and EDX were used to analyze the graft ratio of PLLA-g-BG, the dispersion of nano-particles and the surface elements of the composites respectively. The rabbit osteoblasts were seeded and cultured on the thin films of composites in vitro. The cell adhesion, spreading and growth of osteoblasts were analyzed with FITC staining, NIH Image J software and MTT assay. The change of cell cycle was monitored by flow cytometry(FCM). The results demonstrated that the surface modification of BG with PLLA could significantly improve the dispersing of the particles in the matrix of PLGA. The nanocomposite with 20%PLLA-g-BG exhibited superior surface properties, including roughness and plenty of silicon, calcium and phosper, to enhance the adhesion, spreading and proliferation of osteoblasts. Our results indicate that the application of PLLA-g-BG with a certain blend ratio can improve the bioactivity and biocompatibility of biodegradable polyester.

Key words: Bioglass nanoparticle, Surface grafting, Poly(Lactide-co-glycolide)(PLGA), Nanocomposite, Bioactivity