Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (10): 1987.

• Articles • Previous Articles     Next Articles

Effect of Pretreated Silk Fibroin Films on the Regulation of Hydroxyapatite Crystal Growth

LIU Lin1, KONG Xiang-Dong2, LI Yu-Cheng1, CAI Yu-Rong1, YAO Ju-Ming1*   

  1. 1. Key Laboratory of Advanced Textile Materials and Manufacturing Technology, Ministry of Education, College of Materials and Textile,
    2. College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China
  • Received:2009-03-05 Online:2009-10-10 Published:2009-10-10
  • Contact: YAO Ju-Ming. E-mail: yaoj@zstu.edu.cn
  • Supported by:

    长江学者和创新团队发展计划(批准号: IRT0654)、新世纪优秀人才支持计划(批准号: NCET070763)、国家“九七三”计划前期研究专项(批准号: 2008CB617506)和钱江人才计划(批准号: 2008R10006)资助.

Abstract:

The silk fibroin films were used as the organic templates to induce the deposition of hydroxyapatite(HAP) crystals in the simulate body fluid. The effect of deposition time and different pretreatment methods for silk fibroin film on the formation of HAP crystals was investigated via XRD, SEM, HRTEM, AFM, FTIR, etc. The results indicate that the silk fibroin films can regulate effectively the deposition and growth of HAP crystals. Longer deposition time is apt to form more HAP crystals with well crystallinity. Furthermore, diffe-rent pretreatment methods lead to various morphology and growth direction of HAP crystals according to the rearrangement of silk film′s surface groups and activation of carboxyl and amino groups induce by EDC/NHS. The mechanism for the silk fibroin film regulating the HAP crystal growth was discussed. The study on the effect of pretreated silk fibroin films on the regulation of hydroxyapatite crystal growth will be helpful for further understanding the process of silk fibroin biomineralization.

Key words: Biomineralization; Silk fibroin film; Hydroxyapatite; Simulated body fluid

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