CJCU

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (10): 1980.

• Articles • Previous Articles     Next Articles

Comparison Analysis of Proteome of Both Cerebral and Optic Ganalions in Sepia esculenta

HUANG Fu-Sheng1, CHEN Hai-Bin1, HUANG Lin1, HUANG He-Qing1,2,3*   

  1. 1. Department of Biochemistry and Biotechnology, School of Life Sciences,
    2. State Key Laboratory of Marine Environmental Science, College of Oceanography and Environmental Science,
    3. Key Laboratory of Chemical Biology of Fujian Province, College of Chemistry & Chemical Engineering, Xiamen University, Xiamen 361005, China
  • Received:2009-06-23 Online:2009-10-10 Published:2009-10-10
  • Contact: HUANG He-Qing. E-mail: hqhuang@xmu.edu.cn
  • Supported by:

    国家自然科学基金(批准号： 04776060)和福建省高校创新团队资金资助.

PDF (PC)

Abstract:

Sepia esculenta vests in a cephalopoda animal in mollusca. The differentitation of neural system show not only relative higher level in Cephalopoda animal, but also is easily to separate for studying in the neural sciences. Here, proteomes of both cerebral and optic ganglions in Sepia esculenta were effectively separated by a improved approach of 2D-PAGE. In addition, we used both peptide mass fingerprinting(PMF) and database search to identify protein spots in part in 2D-PAGE gel for establishing maps of molecular anatomic of cerebral and optic ganglions primarily in Sepia esculenta. We used a software of Melanie 4 Trial to analyze those ganglions, indicating approximately 682 and 594 protein spots in cerebral and optic ganglions, respectively, which the spot numbers in front more than that in later. Moreover, the same proteins such as mitochondrial malate dehydrogenase precursor, pre-MDH, SNAP-type proteins can be found by both ganglions. In addition, these spots such as elongation factor G, tubulin, and actin shows high match rate, but most protein identified in the gel are hypothetic and structural proteins.

Key words: Sepia esculenta; Cerebral ganglion; Optic ganglion; Proteomics; Identification

TrendMD: