Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (10): 1976.

• Articles • Previous Articles     Next Articles

Bioassay-guided Isolation of Antitumor Compounds from Derris trifoliate Lour and Their Interaction with DNA

FANG Yu-Chun, SHAO Chang-Lun, LI Jing, LI Yan-Tuan, JIANG Man, WANG Chang-Yun*   

  1. Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
  • Received:2009-01-25 Online:2009-10-10 Published:2009-10-10
  • Contact: WANG Chang-Yun
  • Supported by:

    国家自然科学基金(批准号: 30572314)、国家教育部新世纪优秀人才支持计划(批准号: NCET-05-0600)、国家科技基础性工作专项基金(批准号: 2007FY210500)和山东省优秀中青年科学家科研奖励基金(批准号: 03BS109)资助.

Abstract:

Two antitumor compounds, tephrosin(1) and rotenone(2), were acquied by bioassay-guided isolation from Derris trifoliate Lour. Compounds 1 and 2, assayed by SRB(Sulforhodamine B) methods, showed antitumor activities against cell lines of HCT8, BEL7402, BGC823, A549 and A2780 in vitro with IC50 values from 0.1 to 90.0 μmol/L. The interaction of the compounds with HS-DNA was investigated using UV spectra and fluorescent spectra. The absorbance values at the UV max peaks of compounds 1 and 2 were reduced by adding HS-DNA while the intensity of fluorescent spectra of EB-HS-DNA were gradually weaken following the increased amounts of compounds 1 and 2. It indicated that the mode of antitumor activities of compounds 1 and 2 was relative to binding with DNA through intercalation mode.

Key words: Derris trifoliate Lour; Antitumor compound; Bioassay-guided isolation; DNA interaction

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