Simultaneous Determination of Three Quionlones Residues in Milk by Pyridinium Ionic Liquid-based Aqueous Two-phase Systems Coupled with High Performance Liquid Chromatography

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (10): 1956.

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Simultaneous Determination of Three Quionlones Residues in Milk by Pyridinium Ionic Liquid-based Aqueous Two-phase Systems Coupled with High Performance Liquid Chromatography

ZENG Yan-Bo¹, ZHAO Di-Hai^1,2, LI Lei^1*, WANG Lian³, SHEN Bing³, XI Qi-Hui³, ZHANG Meng-Meng³

1. School of Biological and Chemical Engineering, Jiaxing University, Jiaxing 314001, China;
2. School of Material and Chemical Engineering, Jiangxi University of Technology, Ganzhou 341000, China;
3. Jiaxing Entry-Exit Inspection and Quarantine Bureau, Jiaxing 314001, China

Received:2009-03-09 Online:2009-10-10 Published:2009-10-10
Contact: LI Lei. E-mail: lileichem@yahoo.com.cn
Supported by:
浙江省科技计划(批准号: 2007C23071, 2007F70011)、浙江省自然科学基金(批准号: Y4080252)和嘉兴市科技计划(批准号: 2008AY2016)资助.

Abstract

Abstract:

A method was developed for the analysis of three quinolones(oxolinic acid, nalidix acid and flumequine) in milk by pyridinium ionic liquid-based aqueous two-phase systems coupled with high performance liquid chromatography. Milk samples were extracted with the mixed solutions consisting of sodium chloride and phosphoric acid. Then extracts were enriched by aqueous two-phase systems composed of the ionic liquid pyridine N-ethyl -2-methyl-pyridine bromide salt([EMPy]Br) and K₂HPO₄. The separation of the analytes was achieved via 0.1% phosphoric acid and acetonitrile with gradient elution under UV detection. The linear ranges are 0.3—15 μg/mL for oxolinic acid, 0.5—20 μg/mL for nalidixic acid and 0.5—25 μg/mL for flumequine. The correlation coefficient(r) is more than 0.9997. The limits of detection are 8—10 μg/kg. Based on detecting spiked quinolones concentrations in milk samples, the absolute recoveries ranged from 86.4% to 94.8% with relative standard deviation from 3.6% to 8.3%. The proposed method of determinating quinolones residues in milk is simple, rapid, friendly to environment and high sensitive.

Key words: Aqueous two-phase systems; Pyridinium ionic liquid; High performance liquid chromatography; Determination of quinolone; Milk

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ZENG Yan-Bo, ZHAO Di-Hai, LI Lei*, WANG Lian, SHEN Bing, XI Qi-Hui, .... Simultaneous Determination of Three Quionlones Residues in Milk by Pyridinium Ionic Liquid-based Aqueous Two-phase Systems Coupled with High Performance Liquid Chromatography[J]. Chem. J. Chinese Universities, 2009, 30(10): 1956.

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Simultaneous Determination of Three Quionlones Residues in Milk by Pyridinium Ionic Liquid-based Aqueous Two-phase Systems Coupled with High Performance Liquid Chromatography

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