Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (10): 1951.

• Articles • Previous Articles     Next Articles

HUANG Shan, XIAO Qi, HE Zhi-Ke*, LIU Yi   

  1. College of Chemistry and Molecular Sciences, Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, Wuhan University, Wuhan 430072, China
  • Received:2009-04-28 Online:2009-10-10 Published:2009-10-10
  • Contact: HE Zhi-Ke. E-mail: zhkhe@whu.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 90717111, 20873096, 20621502)资助.

Abstract:

A simple and rapid method for lysozyme determination was developed based on the interaction between CdSe quantum dots and lysozyme. Under the optimized conditions, the linear range of resonance light scattering intensity versus the concentration of lysozyme was 0.01—0.8 μmol/L, with a correlation coefficient of 0.9960. The limit of detection of lysozyme of this method was 5.2 nmol/L. The relative standard deviation for 0.09 μmol/L lysozyme was 2.1%(n=5). The proposed method possessed the advantage of specificity. There was no interference to coexisting foreign substances including common ions, proteins and common amino acids. The synthetic samples were analyzed by the methodology and the results were satisfying. The proposed method has been applied to the determination of lysozyme in synthetic samples. The interaction between CdSe quantum dots and lysozyme was further investigated by using circular dichroism, TEM and fluorescent lifetime. These results indicated that the interaction between CdSe quantum dtos and lysozyme not only resulted in the change of the lysozyme conformation, but also led to the variations of the dispersibility and fluorescent lifetime of CdSe quantum dots.

Key words: Quantum dot; Resonance light scattering; Lysozyme; Circular dichroism; Fluorescent lifetime

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