Studies on Fragmentation Pathways of Amino Acids and Their Interactions with Ginsenoside Rb3 by Spectrospray Ionization Mass Spectrometry

Chem. J. Chinese Universities

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Studies on Fragmentation Pathways of Amino Acids and Their Interactions with Ginsenoside Rb₃ by Spectrospray Ionization Mass Spectrometry

QU Chen-Ling, ZHANG Han-Qi*, ZHANG Hua-Rong, BAI Yu-Ping, WEN Hui

College of Chemistry, Jilin University, Changchun 130012, China

Received:2008-05-16 Revised:1900-01-01 Online:2008-09-10 Published:2008-09-10
Contact: ZHANG Han-Qi

Abstract

Abstract: Electrospray ionization tandem mass spectrometry(MS/MS) was applied to study fragmentation pathways of 18 kinds of α-amino acids in positive and negative ion mode. The groups —NH₂ and —COOH which joined to α-C were found to be more inclined to be fragmented, but not the side chain. The peak intensities of molecular ions in positive ion mode are stronger than those in negative mode. Electrospray ionization mass spectrometry was applied to study the noncovalent interaction of ginsenoside Rb₃ and 18 kinds of amino acids. The dissociation constants were calculated directly based on the peak intensities. It is indicated from the experimental results that the complexes of acidic or alkaline amino acids and ginsenosides are more stable than those of other amino acids.

Key words: Electrospray ionization tandem mass spectrometry, Amino acids, Fragmentation, Ginsenosides, Noncovalent interaction

CLC Number:

O657.6

TrendMD:

QU Chen-Ling, ZHANG Han-Qi*, ZHANG Hua-Rong, BAI Yu-Ping, WEN Hui. Studies on Fragmentation Pathways of Amino Acids and Their Interactions with Ginsenoside Rb₃ by Spectrospray Ionization Mass Spectrometry[J]. Chem. J. Chinese Universities, doi: .

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Studies on Fragmentation Pathways of Amino Acids and Their Interactions with Ginsenoside Rb₃ by Spectrospray Ionization Mass Spectrometry

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