Abstract: To analyze the protein expression changes of a human liver cell lines Huh7 in the absence and presence of HCV, proteomic techniques were used to establish the protein expression profiles. The differentially expressed proteomes between Huh7 cells and Huh7 cells with HCV were profiled and the 14 differentially expressed proteins were identified via two dimensional electrophoresis and MALDI-TOF-MS. Many proteins, included regucalcin, centriolin and several keratins, were up regulated expression after HCV transfection. Among down-regulated expression proteins, heat shock proteins were very attractive. Subsequently, we randomly identified the proteomics results by the detection of Hsp70 expression levels between Huh7 cells and Huh7 cells with HCV by western blot methods. Our study provides the global information of proteomic alteration of Huh7 cells in the presence of intact HCV and the clues for further understanding of the mechanism of interaction between HCV and host cell.

Key words: Cultural cells, Hepatitis C virus, Proteomics