Heat-Induced Changes of Secondary Structures of BSA in D2O Studied by Infrared Spectroscopy and Window Factor Analysis

Chem. J. Chinese Universities

• 研究论文 • Previous Articles Next Articles

Heat-Induced Changes of Secondary Structures of BSA in D₂O Studied by Infrared Spectroscopy and Window Factor Analysis

YUAN Bo*, YAN Hui-Min

State Key Laboratory of Modern Optical Instrumentation, CNERC for Optical Instrument, Zhejiang University, Hangzhou 310027, China

Received:2007-07-02 Revised:1900-01-01 Online:2007-12-10 Published:2007-12-10
Contact: YUAN Bo

Abstract

Abstract: Heat-induced changes of secondary structures of bovine serum albumin(BSA) in D₂O was studied by using infrared spectroscopy and window factor analysis(WFA). The results obtained from the conventional spectral analysis methods and WFA indicate that conformational changes of BSA began at 56 ℃, while the drastic variations of secondary structures occurred in the temperature range of 68—82 ℃. Additionally, the temperature at which the variation of short-segment chains connecting α-helical segment took place is lower by round 10 ℃ than that of the other secondary structures. The present study reveals that WFA plays a key role in the analysis the temperature-dependent infrared spectra of protein in solution.

Key words: Infrared spectroscopy, Window factor analysis(WFA), Heating, Bovine serum albumin(BSA), Secondary structure

CLC Number:

O657.7

TrendMD:

YUAN Bo*, YAN Hui-Min. Heat-Induced Changes of Secondary Structures of BSA in D₂O Studied by Infrared Spectroscopy and Window Factor Analysis[J]. Chem. J. Chinese Universities, doi: .

[1]	LI Rongye, NI Yunxia, LIU Dandan, LI Zhi, CHENG Yuxin, XIA Mingxin, FU Xiaohui. Synthesis and Characterization of Thermoresponsive Polypeptide/polypeptoid Block Copolymers [J]. Chem. J. Chinese Universities, 2021, 42(3): 850.
[2]	ZHAO Hongtao, SUN Yan, GUO Yichang, CAI Wensheng, SHAO Xueguang. Near Infrared Spectroscopy for Low-temperature Water Structure Analysis [J]. Chem. J. Chinese Universities, 2020, 41(9): 1968.
[3]	HAO Yuanyuan, WU Qi, LI Ji, GE Chao, MA Chaoying, QIAN Yong, SU Zhi, LIU Hongke. Novel Os^Ⅱ-arene Complexes Based on Bipyridyl Derivative Ligands: Synthesis, Crystal Structure, Anticancer Activity and Interaction with DNA/BSA^† [J]. Chem. J. Chinese Universities, 2018, 39(4): 614.
[4]	WANG Linxiang. Preparation and Luminescence Properties of Na⁺, Li⁺, Bi³⁺Doped CaWO₄:Eu³⁺ Phosphors^† [J]. Chem. J. Chinese Universities, 2018, 39(1): 25.
[5]	XU Guoqing, HAO Changchun, HE Jianzhen, ZHANG Lei, SUN Runguang. Effect of Bovine Serum Albumin on the Structure of DSPE Monolayer^† [J]. Chem. J. Chinese Universities, 2017, 38(12): 2238.
[6]	LIU Yang, FU Xianwei, ZHAO Tianyu, LIAN Gang, DONG Ning, SONG Side, WANG Qilong, CUI Deliang. Investigation on Stability of Perovskite Semiconductor CH₃NH₃PbI₃ by In-situ FTIR Spectroscopy^† [J]. Chem. J. Chinese Universities, 2016, 37(9): 1605.
[7]	TONG Laga, REN Guangsheng, DANG Xiaofeng, LIN Shijing, RONG Hua. Induced Growth of Micro/nano ZnO Powder via Glu-BF₄Ionic Liquid Aqueous Solution^† [J]. Chem. J. Chinese Universities, 2016, 37(11): 1939.
[8]	ZHANG Fudong, LIU Jie, WANG Zhihong. Analysis of Oil Yield from Oil Shale Minerals Based on Near-infrared Spectroscopy with Least Squares Support Vector Machines^† [J]. Chem. J. Chinese Universities, 2016, 37(10): 1792.
[9]	ZHANG Jing, CHEN Weixiao, ZHANG Wei, DUAN Ying, ZHU Yuxiu, ZHU Yaxian, ZHANG Yong. Interaction of 1-Hydroxypyrene with BSA Using Fluorescence Anisotropy and Synchronous Fluorescence Analysis Methods^† [J]. Chem. J. Chinese Universities, 2015, 36(8): 1511.
[10]	YANG Shuilan, SONG Pan, SHE Wenjie, YANG Tianlin. Mechanism of the Interaction Between a Phosphorus-containing Tripod Ligand Europium(Ⅲ) Complex and Bovine Serum Albumin^† [J]. Chem. J. Chinese Universities, 2015, 36(7): 1254.
[11]	FAN Xiaokun, JIANG Ye, SHI Jiajia, LIU Cuige, WEI Yongju, SONG Zengfu, WENG Shifu, YANG Zhanlan, XU Yizhuang, WU Jinguang. Sulfur Fine Particles as Stationary Phase for TLC/FTIR Analysis^† [J]. Chem. J. Chinese Universities, 2014, 35(4): 741.
[12]	LIU Xiaoxia, DENG Hao, WANG Yanying, LU Zhiwei, ZENG Xianyin, WANG Xianxiang, ZOU Ping, RAO Hanbing. Thermodynamics Studies on the BSA Adsorption onto Zinc Oxide Surfaces with Different Morphologies^† [J]. Chem. J. Chinese Universities, 2014, 35(10): 2156.
[13]	GUAN Hongyan, LIAN Fang, WEN Yan, PAN Xiaorong, SUN Jialin. Thermal Stability of Novel Polyvinyl Formal Based Gel Polymer Electrolyte^† [J]. Chem. J. Chinese Universities, 2014, 35(1): 80.
[14]	ZHU Ying-Hong, LU Zai-Xiang, CHEN Qiong, MA Chun-An. Direct Electro-oxidation of Toluene Derivations on Graphite Electrode [J]. Chem. J. Chinese Universities, 2013, 34(9): 2210.
[15]	LIN Hai-Bin, ZHENG Lin, LIN Yu-Qin, ZHOU Zhao-Hui. Study of Interaction Between Bovine Serum Albumin and Cobalt Complexes of Phenanthroline and Nitrilotriacetate by Fluorescence Spectrometry [J]. Chem. J. Chinese Universities, 2013, 34(8): 1818.

Heat-Induced Changes of Secondary Structures of BSA in D₂O Studied by Infrared Spectroscopy and Window Factor Analysis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments