Abstract: In 0.12 mol/L hydrochloric acid and nitric acid media, mitoxantrone interacted with molybdate to form an ion-association complex, which can result in the significant enhancement of RRS intensity. The maximum scattering wavelengths were 365 nm for RRS and scattering intensities are proportional to the concentration of mitoxantrone, which can be applied to the determination of mitoxantrone. The detection limits are 6 ng/mL in nitric acid and 8 ng/mL in hydrochloric acid respectively. Because there are not such interactions between anthracycline anticancer drugs such as daunorubicin and epirubicin with molybdate, mitoxantrone can be determined selectively with other anthracycline anticancer drugs existing. The optimum experiment condition, affecting factor and the effect of coexisting substance were investigated in the paper. The application to the determination of mitoxantrone in serum and urine by RRS were not only sensitive but also simple and ra-pid. In addition, the interaction mechanism was discussed.

Key words: Mitoxantrone, Ammonium molybdate, Resonance Rayleigh scattering